Detection of carrier cattle and sheep persistently infected with foot-and-mouth disease virus by a rapid real-time RT-PCR assay

被引：43

作者：

Zhang, ZD ^{[1
]}

Alexandersen, S ^{[1
]}

机构：

[1] AFRC, Inst Anim Hlth, Pirbright Lab, Woking GU24 0NF, Surrey, England

来源：

JOURNAL OF VIROLOGICAL METHODS | 2003年 / 111卷 / 02期

关键词：

real-time RT-PCR; foot-and-mouth disease virus; probang samples; carriers; persistence;

D O I：

10.1016/S0166-0934(03)00165-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The detection of foot-and-mouth disease virus (FMDV) in persistently infected carriers among exposed ruminants is of great importance in disease control. For this purpose, a real time, fluorogenic reverse transcription polymerase chain reaction (real-time RT-PCR) assay was evaluated for the identification of FMDV carrier animals. The results indicate that this real time RT-PCR assay may be suitable for detection of FMDV carrier animals. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：95 / 100

页数：6

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