A highly selective fluorescence turn-on sensor for Cysteine/Homocysteine and its application in bioimaging

被引：499

作者：

Zhang, Meng

Yu, Mengxiao

Li, Fuyou ^{[1
]}

Zhu, Minwei

Li, Manyu

Gao, Yanhong

Li, Lei

Liu, Zhiqiang

Zhang, Jianping

Zhang, Dengqing

Yi, Tao

Huang, Chunhui

机构：

[1] Fudan Univ, Dept Chem, Shanghai 200433, Peoples R China

[2] Fudan Univ, Adv Mat Lab, Shanghai 200433, Peoples R China

[3] Fudan Univ, Hosp Obstet & Gynecol, Shanghai 200433, Peoples R China

[4] Chinese Acad Sci, Inst Chem, State Key Lab Struct Chem Unstable & Stable Speci, Beijing 100080, Peoples R China

[5] Shanghai Jiao Tong Univ, Hosp Xinhua, Sch Med, Shanghai 200092, Peoples R China

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 34期

关键词：

D O I：

10.1021/ja073140i

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We demonstrated a highly electron-deficient system 1 as a turn-on fluorescence sensor for intracellular imaging of Cys/Hcy in biological samples. On the basis of the specific nucleophilic ability of thiols of Cys and Hcy, 1 exhibited high selectivity and sensitivity for Cys/Hcy over other amino acids and thiol biomolecules. This probe is the first Cys/Hcy sensor with excitation in the visible region and turn-on (75-fold) fluorescence emission and also gives a significant increase in two-photon excited fluorescence for sensing Cys/Hcy. Moreover, confocal laser scanning microscopy and two-photon laser scanning microscopy experiments further established that 1 can be used for sensing Cys/Hcy within biological samples.

引用

页码：10322 / +

页数：3

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