Gene silencing in Caenorhabditis elegans by transitive RNA interference

被引：80

作者：

Alder, MN ^{[1
]}

Dames, S ^{[1
]}

Gaudet, J ^{[1
]}

Mango, SE ^{[1
]}

机构：

[1] Univ Utah, Huntsmann Canc Inst, Salt Lake City, UT 84112 USA

来源：

RNA | 2003年 / 9卷 / 01期

关键词：

RNA interference; transitive RNAi; C; elegans; pha-4; RdRP;

D O I：

10.1261/rna.2650903

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

When a cell is exposed to double-stranded RNA (dsRNA), mRNA from the homologous gene is selectively degraded by a process called RNA interference (RNAi). Here, we provide evidence that dsRNA is amplified in Caenorhabditis elegans to ensure a robust RNAi response. Our data suggest a model in which mRNA targeted by RNAi functions as a template for 5' to 3' synthesis of new dsRNA (termed transitive RNAi). Strikingly, the effect is nonautonomous: dsRNA targeted to a gene expressed in one cell type can lead to transitive RNAi-mediated silencing of a second gene expressed in a distinct cell type. These data suggest dsRNA synthesized in vivo can mediate systemic RNAi.

引用

页码：25 / 32

页数：8

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