High-pressure solution X-ray scattering of protein using a hydrostatic cell with diamond windows

被引：23

作者：

Kato, M ^{[1
]}

Fujisawa, T

机构：

[1] Ritsumeikan Univ, Fac Sci & Engn, Dept Chem, Shiga 52577, Japan

[2] RIKEN, Inst Phys & Chem Res, Struct Biophys Lab, Harima Inst, Ako, Hyogo 67812, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 1998年 / 5卷

关键词：

high pressure; quantitative solution X-ray scattering; hydrostatic cells; lysozyme; radius of gyration;

D O I：

10.1107/S0909049598000788

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A hydrostatic high-pressure cell (maximum pressure 700 MPa) with synthetic diamond windows is applied to measure small-angle X-ray scattering of a protein at high pressure. Use of the present fell allows an accurate solvent background correction, providing quantitative analyses. The performance of the present cell for X-ray scattering is shown by using lysozyme as a sample solution. From the Guinier plot, values of the radius of gyration of lysozyme are evaluated to be 15.31 +/- 0.09 Angstrom at I atm (0.10 MPa) and 14.80 +/- 0.15 Angstrom at 400 MPa.

引用

页码：1282 / 1286

页数：5

共 31 条

[1] THERMODYNAMICS OF PROTEIN DENATURATION - EFFECT OF PRESSURE ON DENATURATION OF RIBONUCLEASE-A [J].

BRANDTS, JF ;

OLIVEIRA, RJ ;

WESTORT, C .

BIOCHEMISTRY, 1970, 9 (04) :1038-&

[2]

Bridgman PW, 1914, J BIOL CHEM, V19, P511

[3] ASSOCIATION-DISSOCIATION PROPERTIES OF LYSOZYME [J].

BRUZZESI, MR ;

CHIANCONE, E ;

ANTONINI, E .

BIOCHEMISTRY, 1965, 4 (09) :1796-+

[4] High pressure synchrotron X-ray diffraction studies of biological molecules using the diamond anvil technique [J].

Czeslik, C ;

Malessa, R ;

Winter, R ;

Rapp, G .

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION A-ACCELERATORS SPECTROMETERS DETECTORS AND ASSOCIATED EQUIPMENT, 1996, 368 (03) :847-851

[5] THEORY OF THE GASKET IN DIAMOND ANVIL HIGH-PRESSURE CELLS [J].

DUNSTAN, DJ .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1989, 60 (12) :3789-3795

[6]

DUNSTAN DJ, 1989, J PHYS E, V22, P923

[7] EQUIPMENT FOR HIGH PRESSURE OPTICAL AND SPECTROSCOPIC STUDIES [J].

FISHMAN, E ;

DRICKAMER, HG .

ANALYTICAL CHEMISTRY, 1956, 28 (05) :804-806

[8] THE HYDRATION OF RAS P21 IN SOLUTION DURING GTP HYDROLYSIS BASED ON SOLUTION X-RAY-SCATTERING PROFILE [J].

FUJISAWA, T ;

URUGA, T ;

YAMAIZUMI, Z ;

INOKO, Y ;

NISHIMURA, S ;

UEKI, T .

JOURNAL OF BIOCHEMISTRY, 1994, 115 (05) :875-880

[9] REVERSIBLE PRESSURE-TEMPERATURE DENATURATION OF CHYMOTRYPSINOGEN [J].

HAWLEY, SA .

BIOCHEMISTRY, 1971, 10 (13) :2436-&

[10] A HYDROSTATIC OPTICAL-CELL WITH SYNTHETIC DIAMOND WINDOWS FOR QUANTITATIVE INFRARED MEASUREMENTS OF FLUIDS [J].

KATO, M ;

TANIGUCHII, Y .

REVIEW OF SCIENTIFIC INSTRUMENTS, 1995, 66 (08) :4333-4335

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