High-pressure solution X-ray scattering of protein using a hydrostatic cell with diamond windows

被引：23

作者：

Kato, M ^{[1
]}

Fujisawa, T

机构：

[1] Ritsumeikan Univ, Fac Sci & Engn, Dept Chem, Shiga 52577, Japan

[2] RIKEN, Inst Phys & Chem Res, Struct Biophys Lab, Harima Inst, Ako, Hyogo 67812, Japan

来源：

JOURNAL OF SYNCHROTRON RADIATION | 1998年 / 5卷

关键词：

high pressure; quantitative solution X-ray scattering; hydrostatic cells; lysozyme; radius of gyration;

D O I：

10.1107/S0909049598000788

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

A hydrostatic high-pressure cell (maximum pressure 700 MPa) with synthetic diamond windows is applied to measure small-angle X-ray scattering of a protein at high pressure. Use of the present fell allows an accurate solvent background correction, providing quantitative analyses. The performance of the present cell for X-ray scattering is shown by using lysozyme as a sample solution. From the Guinier plot, values of the radius of gyration of lysozyme are evaluated to be 15.31 +/- 0.09 Angstrom at I atm (0.10 MPa) and 14.80 +/- 0.15 Angstrom at 400 MPa.

引用

页码：1282 / 1286

页数：5

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