High-density culture of recombinant Chinese hamster ovary cells producing prothrombin in protein-free medium

被引：13

作者：

Chen, ZL

Lütkemeyer, D

Iding, K

Lehmann, J

机构：

[1] Inst Biotechnol, Dept Cell Engn, Beijing 100071, Peoples R China

[2] Univ Bielefeld, Inst Cell Culture Technol, D-33501 Bielefeld, Germany

来源：

BIOTECHNOLOGY LETTERS | 2001年 / 23卷 / 10期

关键词：

high-density cultivation; protein-free medium; prothrombin; recombinant CHO cell;

D O I：

10.1023/A:1010373527375

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A recombinant CHO cell line, CHO2DS, was immobilized on porous microcarrier Cytopore 1 and cultivated in 1 l modified Super-spinner and 2 l stirred tank bioreactor with the perfusion of a low-cost chemically defined protein-free medium DF6S. CHO2DS cells could enter into the inner space and grew both in the inner space and on the surface of Cytopore 1 in DF6S and produced prothrombin at 22 mg l(-1) after 10 days. From a seeding density of 5.7 x 10(5) cells ml(-)1, the highest viable cell density of CHO2DS was 1.12 x 10(7) cells ml(-1).

引用

页码：767 / 770

页数：4

共 11 条

[1] A low-cost chemically defined protein free medium for a recombinant CHO cell line producing prothrombin [J].