Coexpression and regulation of the FGF-2 and FGF antisense genes in leukemic cells

被引：22

作者：

Baguma-Nibasheka, M

Li, AW

Osman, MS

Geldenhuys, L

Casson, AG

Too, CKL

Murphy, PR

机构：

[1] Dalhousie Univ, Fac Med, Dept Physiol & Biophys, Halifax, NS B3H 1X5, Canada

[2] Dalhousie Univ, Fac Med, Dept Biochem & Mol Biol, Halifax, NS B3H 1X5, Canada

[3] Dalhousie Univ, Fac Med, Dept Pathol, Halifax, NS B3H 1X5, Canada

[4] Dalhousie Univ, Fac Med, Dept Surg, Halifax, NS B3H 1X5, Canada

[5] Dalhousie Univ, Fac Med, Dept Obstet & Gynaecol, Halifax, NS B3H 1X5, Canada

来源：

LEUKEMIA RESEARCH | 2005年 / 29卷 / 04期

基金：

加拿大健康研究院;

关键词：

fibroblast growth factor; FGF-2; antisense; lymphoma; nuclear;

D O I：

10.1016/j.leukres.2004.09.006

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Fibroblast growth factor-2 (FGF-2) is a growth and survival factor whose expression is elevated in many hematopoietic malignancies. A natural antisense RNA (FGF-AS) has been implicated in the posttranscriptional regulation of FGF-2 mRNA expression. We demonstrate for the first time that FGF sense and antisense RNAs are coordinately expressed and translated in hematopoietic cells and tissues. Cytokine stimulation of growth-arrested K562 cells elicited a rapid transient increase in FGF-AS mRNA expression followed by a slower but sustained increase in FGF-2 mRNA. This was accompanied by a marked increase in the expression and nuclear translocation of FGF-2 and the FGF-AS encoded protein, GFG/NUDT6. These findings suggest a role for both FGF-2 and GFG proteins in the cell survival and proliferation of lymphoid and myeloid tumor cells. (c) 2004 Elsevier Ltd. All rights reserved.

引用

页码：423 / 433

页数：11

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[1] The NADH diphosphatase encoded by the Saccharomyces cerevisiae NYP1 nudix hydrolase gene is located in peroxisomes [J].