Speed-mapping quantitative trait loci using microarrays

被引:36
作者
Lai, Chao-Qiang
Leips, Jeff
Zou, Wei
Roberts, Jessica F.
Wollenberg, Kurt R.
Parnell, Laurence D.
Zeng, Zhao-Bang
Ordovas, Jose M.
Mackay, Trudy F. C.
机构
[1] Univ Maryland Baltimore Cty, Dept Biol Sci, Baltimore, MD 21250 USA
[2] PGxHlth, New Haven, CT 06511 USA
[3] Tufts Univ, New England Med Ctr, Boston, MA 02111 USA
[4] NCI, NIAID, Bioinformat & Sci IT Program, Off Technol Informat Syst, Bethesda, MD 20892 USA
[5] Tufts Univ, USDA Human Nutr Res Ctr Aging Nutr & Genom, Boston, MA 02111 USA
[6] N Carolina State Univ, WM Keck Behav Biol, Dept Genet, Raleigh, NC 27695 USA
[7] N Carolina State Univ, Bioinformat Res Ctr, Raleigh, NC 27695 USA
关键词
D O I
10.1038/NMETH1084
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
We developed a rapid, economical method for high-resolution quantitative trait locus ( QTL) mapping using microarrays for selective genotyping of pooled DNA samples. We generated 21,207 F-2 flies from two inbred Drosophila melanogaster strains with known QTLs affecting lifespan, and hybridized DNA pools of young and old flies to microarrays. We used changes of gene frequency of 2,326 single-feature polymorphisms ( SFPs) to map previously identified and additional QTLs affecting lifespan.
引用
收藏
页码:839 / 841
页数:3
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