Poly(hydroxybutyrate-co-hydroxyhexanoate) promoted production of extracellular matrix of articular cartilage chondrocytes in vitro

被引:107
作者
Deng, Y
Lin, XS
Zheng, Z
Deng, JG
Chen, JC
Ma, H
Chen, GQ [1 ]
机构
[1] Tsing Hua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Tsing Hua Univ, Dept Phys, Beijing 100084, Peoples R China
关键词
polyhydroxyalkanoates; PHB; type II collagen; SHG; chondrocytes;
D O I
10.1016/S0142-9612(03)00367-3
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
The present investigation describes the production of extracellular matrix of rabbit articular cartilage chondrocytes grown on scaffolds of polyhydroxybutyrate (PHB) blended with poly(hydroxybutyrate-co-hydroxyhexanoate) (PHBHHx) for up to 7 days. The mRNA level of type II collagen of chondrocytes seeded on all scaffolds consisting of PHBHHx were obviously higher than that of PHB-only scaffold throughout the culture period, suggesting the positive effect of PHBHHx on extracellular matrix production. Second-harmonic generation (SHG) imaging technique, combined with confocal fluorescence microscopy (CFM) revealed that PHBHHx in PHB scaffold provided better surface properties for anchoring type II collagen filaments and their penetration into internal layers of the scaffolds. Glycosaminoglycan (GAG), a major composition of extracellular matrix, showed a sharp increase in construct of 1:2 PHB/PHBHHx scaffold after 7 day cultivation, while only a small increase was observed in all other tested scaffolds. At the same time, total collagen contents in all scaffolds containing PHBHHx increased with time, with the maximum collagen production of 742.1 +/- 99.2 mg/g dry weight observed in construct of 1:2 PHB/PHBHHx scaffold inoculated for 7 days, this was almost 4-fold higher than that in scaffold of PHB only. It appears that the presence of right proportion of PHBHHx in the composite system of PHB/PHBHHx highly favored the production of extracellular matrix of articular cartilage chondrocytes. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:4273 / 4281
页数:9
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