Regulation of myosin heavy chain expression in adult rat hindlimb muscles during short-term paralysis: Comparison of denervation and tetrodotoxin-induced neural inactivation

被引:46
作者
Michel, RN [1 ]
Parry, DJ [1 ]
Dunn, SE [1 ]
机构
[1] UNIV OTTAWA,FAC MED,DEPT PHYSIOL,OTTAWA,ON K1H 8M5,CANADA
基金
加拿大自然科学与工程研究理事会; 英国医学研究理事会;
关键词
myosin; immunohistochemistry; activity; trophic mechanism; embryonic MHC; alpha-cardiac MHC;
D O I
10.1016/0014-5793(96)00618-7
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The extent to which myosin profiles within adult fast and slow muscles are altered by short-term paralysis remains equivocal. We used an array of specific antibodies to identify adult and developmental MHC isoforms within EDL and soleus muscle fibers, and show a marked multiple expression of MHCs with a general shift towards slower and more energy efficient MHC profiles after 2 weeks of denervation or TTX nerve conduction block, Paralysis also induced marked expression of an embryonic MHC within most EDL cell types, and a subtle, paralysis-sensitive, expression of alpha-cardiac MHC within specific EDL and soleus extrafusal fibers, Comparison of treatment groups also permitted assessment of the relative influence of neural activity versus trophic factors on these isoforms, and confirmed activity as a major, but not sole, regulator of MHC expression.
引用
收藏
页码:39 / 44
页数:6
相关论文
共 41 条
  • [1] EMG ACTIVITY OF SLOW AND FAST ANKLE EXTENSORS FOLLOWING SPINAL-CORD TRANSECTION
    ALAIMO, MA
    SMITH, JL
    ROY, RR
    EDGERTON, VR
    [J]. JOURNAL OF APPLIED PHYSIOLOGY, 1984, 56 (06) : 1608 - 1613
  • [2] ALAMOOD WS, 1989, J PHYSIOL-LONDON, V414, P1
  • [3] AUSONI S, 1990, J NEUROSCI, V10, P153
  • [4] BAKER AJ, 1983, J PHYSIOL-LONDON, V345, pP56
  • [5] MYOFIBRILLAR ATPASE ACTIVITY DURING ISOMETRIC CONTRACTION AND ISOMYOSIN COMPOSITION IN RAT SINGLE SKINNED MUSCLE-FIBERS
    BOTTINELLI, R
    CANEPARI, M
    REGGIANI, C
    STIENEN, GJM
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1994, 481 (03): : 663 - 675
  • [6] UNLOADED SHORTENING VELOCITY AND MYOSIN HEAVY-CHAIN AND ALKALI LIGHT-CHAIN ISOFORM COMPOSITION IN RAT SKELETAL-MUSCLE FIBERS
    BOTTINELLI, R
    BETTO, R
    SCHIAFFINO, S
    REGGIANI, C
    [J]. JOURNAL OF PHYSIOLOGY-LONDON, 1994, 478 (02): : 341 - 349
  • [7] CARRARO U, 1990, DYNAMIC STATE OF MUSCLE FIBERS, P247
  • [8] THE EFFECT OF DENERVATION ON MYOSIN ISOFORM SYNTHESIS IN RABBIT SLOW-TYPE AND FAST-TYPE MUSCLES DURING TERMINAL DIFFERENTIATION - DENERVATION INDUCES DIFFERENTIATION INTO SLOW-TYPE MUSCLES
    DALBIS, A
    GOUBEL, F
    COUTEAUX, R
    JANMOT, C
    MIRA, JC
    [J]. EUROPEAN JOURNAL OF BIOCHEMISTRY, 1994, 223 (01): : 249 - 258
  • [9] MYOTROPIC EFFECTS ON DENERVATED FAST-TWITCH MUSCLES OF MICE - CORRELATION OF PHYSIOLOGIC, BIOCHEMICAL, AND MORPHOLOGIC FINDINGS
    DAVIS, HL
    BRESSLER, BH
    JASCH, LG
    [J]. EXPERIMENTAL NEUROLOGY, 1988, 99 (02) : 474 - 489
  • [10] MYOSIN HEAVY-CHAIN EXPRESSION IN HUMAN-MUSCLE COCULTURED WITH MOUSE SPINAL-CORD
    ECOBPRINCE, M
    HILL, M
    BROWN, W
    [J]. JOURNAL OF THE NEUROLOGICAL SCIENCES, 1989, 90 (02) : 167 - 177