Spatiotemporal Control of Lipid Conversion, Actin-Based Mechanical Forces, and Curvature Sensors during Clathrin/AP-1-Coated Vesicle Biogenesis

被引：22

作者：

Anitei, Mihaela ^{[1
]}

Stange, Christoph ^{[1
]}

Czupalla, Cornelia ^{[1
]}

Niehage, Christian ^{[1
,6
]}

Schuhmann, Kai ^{[2
]}

Sala, Pia ^{[3
,4
]}

Czogalla, Aleksander ^{[3
,4
,5
]}

Pursche, Theresia ^{[1
]}

Coskun, Uenal ^{[3
,4
]}

Shevchenko, Andrej ^{[2
]}

Hoflack, Bernard ^{[1
]}

机构：

[1] Tech Univ Dresden, Biotechnol Ctr, Tatzberg 47-49, D-01307 Dresden, Germany

[2] Max Planck Inst Mol Cell Biol & Genet, Pfotenhauerstr 108, D-01307 Dresden, Germany

[3] Univ Clin Carl Gustav Carus, TU Dresden, Paul Langerhans Inst Dresden, Helmholtz Ctr Munich, D-01307 Dresden, Germany

[4] DZD eV, German Ctr Diabet Res, D-85764 Neuherberg, Germany

[5] Univ Wroclaw, Fac Biotechnol, Dept Cytobiochem, Joliot Curie 14a, PL-50383 Wroclaw, Poland

[6] Mannheim Univ Appl Sci, Instrumental Anal & Bioanal, Paul Wittsack Str 10, D-68163 Mannheim, Germany

来源：

CELL REPORTS | 2017年 / 20卷 / 09期

关键词：

TRANS-GOLGI NETWORK; MANNOSE 6-PHOSPHATE RECEPTOR; ADP-RIBOSYLATION FACTOR; PROTEIN-KINASE-C; PLASMA-MEMBRANE; PHOSPHOLIPASE-D; LIVING CELLS; ARFAPTIN; TRAFFICKING; SCISSION;

D O I：

10.1016/j.celrep.2017.08.013

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Clathrin/adaptor protein-1-coated carriers connect the secretory and the endocytic pathways. Carrier biogenesis relies on distinct protein networks changing membrane shape at the trans-Golgi network, each regulating coat assembly, F-actin-based mechanical forces, or the biophysical properties of lipid bilayers. How these different hubs are spatiotemporally coordinated remains largely unknown. Using in vitro reconstitution systems, quantitative proteomics, and lipidomics, as well as in vivo cell-based assays, we characterize the protein networks controlling membrane lipid composition, membrane shape, and carrier scission. These include PIP5K1A and phospholipase C-beta 3 controlling the conversion of PI[4]P into diacylglycerol. PIP5K1A binding to RAC1 provides a link to F-actin-based mechanical forces needed to tubulate membranes. Tubular membranes then recruit the BAR-domain-containing arfaptin-1/2 guiding carrier scission. These findings provide a framework for synchronizing the chemical/biophysical properties of lipid bilayers, F-actinbased mechanical forces, and the activity of proteins sensing membrane shape during clathrin/adaptor protein-1-coated carrier biogenesis.

引用

页码：2087 / 2099

页数：13

共 64 条

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End-Product Diacylglycerol Enhances the Activity of PI-PLC through Changes in Membrane Domain Structure [J].