Co does not replace Ni with respect to urease activity in zucchini (Cucurbita pepo convar. giromontiina) and soybean (Glycine max)

被引:12
作者
Gerendás, J [1 ]
Polacco, JC
Freyermuth, SK
Sattelmacher, B
机构
[1] Univ Kiel, Inst Plant Nutr & Soil Sci, D-24118 Kiel, Germany
[2] Univ Missouri, Dept Biochem, Columbia, MO 65211 USA
关键词
cobalt; nickel; soybean; substitution; urease activity; zucchini;
D O I
10.1023/A:1004343416511
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
In order to evaluate whether Co can functionally replace Ni in urease, as suggested by Watanabe et al. (Soil Sci. Plant Nutr. (1994) 40, 545-548), zucchini and soybean plants were grown in purified nutrient solutions containing nitrate and ammonium as sole N source supplemented either with 0.05 mmol m(-3) Ni or Co or with no supplementation (control). In addition, isolated soybean cotyledons were incubated axenically with either 1 mmol m(-3) Ni or Co supplements. Plant growth was not affected by the Ni and Co additions, but urease activity was only detected in Ni-supplemented tissue, and in no case did Co raise the urease activity level. Only Ni-deprived plants (-Ni - Co and -Ni + Co) accumulated appreciable amounts of urea in the leaves. These results strongly suggest, that Co does not replace Ni with respect to functional urease.
引用
收藏
页码:127 / 135
页数:9
相关论文
共 33 条
  • [21] A NEW MUTANT CLASS OF SOYBEAN LACKS UREASE IN LEAVES BUT NOT IN LEAF-DERIVED CALLUS OR IN ROOTS
    POLACCO, JC
    JUDD, AK
    DYBING, JK
    CIANZIO, SR
    [J]. MOLECULAR & GENERAL GENETICS, 1989, 217 (2-3): : 257 - 262
  • [22] ROLES OF UREASE IN PLANT-CELLS
    POLACCO, JC
    HOLLAND, MA
    [J]. INTERNATIONAL REVIEW OF CYTOLOGY - A SURVEY OF CELL BIOLOGY, VOL 145, 1993, 145 : 65 - 103
  • [23] Robinson J.B., 1986, PLANT ANAL INTERPRET
  • [24] SAS Institute INC, 1988, SAS STAT US GUID REL
  • [25] SCHNEGG A, 1976, INT J VITAM NUTR RES, V46, P96
  • [26] SCHNEGG HA, 1976, ARCH TIERERNAHR, V26, P543
  • [27] UREASE IS NOT ESSENTIAL FOR UREIDE DEGRADATION IN SOYBEAN
    STEBBINS, NE
    POLACCO, JC
    [J]. PLANT PHYSIOLOGY, 1995, 109 (01) : 169 - 175
  • [28] INVITRO CULTURE OF IMMATURE COTYLEDONS OF SOYA BEAN (GLYCINE-MAX-L-MERR)
    THOMPSON, JF
    MADISON, JT
    MUENSTER, AME
    [J]. ANNALS OF BOTANY, 1977, 41 (171) : 29 - 39
  • [29] EFFECTS OF NI DEFICIENCY ON SOME NITROGEN METABOLITES IN COWPEAS (VIGNA-UNGUICULATA-L WALP)
    WALKER, CD
    GRAHAM, RD
    MADISON, JT
    CARY, EE
    WELCH, RM
    [J]. PLANT PHYSIOLOGY, 1985, 79 (02) : 474 - 479
  • [30] NICKEL ENZYMES
    WALSH, CT
    ORMEJOHNSON, WH
    [J]. BIOCHEMISTRY, 1987, 26 (16) : 4901 - 4906