Smooth muscle length-dependent PI(4,5)P2 synthesis and paxillin tyrosine phosphorylation

被引:11
作者
Sul, D [1 ]
Baron, CB [1 ]
Broome, R [1 ]
Coburn, RF [1 ]
机构
[1] Univ Penn, Sch Med, Dept Physiol, Philadelphia, PA 19104 USA
来源
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY | 2001年 / 281卷 / 01期
关键词
smooth muscle mechanical strain; airway smooth muscle; phosphatidylinositol 4,5-bisphosphate; integrins; phosphatidylinositol; 4-kinase;
D O I
10.1152/ajpcell.2001.281.1.C300
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We studied effects of increasing the length of porcine trachealis muscle on 5.5 muM carbachol (CCh)-evoked phosphatidylinositol 4,5-bisphosphate [PI(4,5) P-2] synthesis and other parameters of phosphatidylinositol (PI) turnover. PI(4,5) P-2 resynthesis rates in muscle held at 1.0 optimal length (L-o), measured over the first 6 min of CCh stimulation, were 140 +/- 12 and 227 +/- 14% of values found in muscle held at 0.5 L-o and in free-floating muscle, respectively. Time-dependent changes in cellular masses of PI(4,5) P-2, PI, and phosphatidic acid, and PI resynthesis rates, were also altered by the muscle length at which contraction occurred. In free-floating muscle, CCh did not evoke increases in tyrosine-phosphorylated paxillin (PTyr-paxillin), an index of beta (1)-integrin signaling; however, there were progressive increases in PTyr-paxillin in muscle held at 0.5 and 1.0 L-o during contraction, which correlated with increases in PI(4,5) P-2 synthesis rates. These data indicate that PI(4,5) P-2 synthesis rates and other parameters of CCh-stimulated inositol phospholipid turnover are muscle length-dependent and provide evidence that supports the hypothesis that length-dependent beta (1)-integrin signals may exert control on CCh-activated PI(4,5)P-2 synthesis.
引用
收藏
页码:C300 / C310
页数:11
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