Ovarian follicular concentrations of activin, follistatin, inhibin, insulin-like growth factor I (IGF-I), IGF-II, IGF-Binding protein-2 (IGFBP-2), IGFBP-3, and vascular endothelial growth factor in spontaneous menstrual cycles of normal women of advanced reproductive age

被引:71
作者
Klein, NA
Battaglia, DE
Woodruff, TK
Padmanabhan, V
Giudice, LC
Bremner, WJ
Soules, MR
机构
[1] Univ Washington, Seattle, WA 98105 USA
[2] Northwestern Univ, Chicago, IL 60208 USA
[3] Univ Michigan, Ann Arbor, MI 48109 USA
[4] Stanford Univ, Stanford, CA 94305 USA
关键词
D O I
10.1210/jc.85.12.4520
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Previous studies indicate that the menstrual cycles of older reproductive age women are characterized by a selective elevation of FSH associated with early development and ovulation of a dominant follicle. Several intraovarian hormones and growth factors have been identified that appear to serve important paracrine roles. The purpose of this study was to examine follicular fluid (FF) hormones and growth factors in the dominant follicle of unstimulated cycles of older, ovulatory women. We aspirated FF from the preovulatory dominant follicle in natural menstrual cycles of older subjects (age, 40-45 yr; n = 20) and younger controls (age, 20-25 yr; n = 19). FF was analyzed for estradiol, progesterone, testosterone, androstenedione, inhibin A and B, total activin A, total follistatin, insulin-like growth factor I (IGF-I), IGF-II, IGF-binding protein-2 (IGFBP-2), IGFBP-3, and vascular endothelial growth factor (VEGF) concentrations. We found that the dominant follicles from older women contain normal concentrations of steroids, inhibin A and B, IGF-II, IGFBP-2, and IGFBP-3; increased concentrations of follistatin, activin A, and VEGF; and decreased concentrations of IGF-I. Therefore, under the influence of elevated FSH, the dominant follicle in older women is highly competent in terms of hormone and growth factor secretion. We postulate that elevated FF activin may be related to the early ovulation observed in older women, whereas elevated VEGF may be related to the meiotic spindle abnormalities observed in the oocytes of older reproductive age women.
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页码:4520 / 4525
页数:6
相关论文
共 39 条
[1]   SIMPLE METHOD FOR ASSAY OF 8 STEROIDS IN SMALL VOLUMES OF PLASMA [J].
ANDERSON, DC ;
HOPPER, BR ;
LASLEY, BL ;
YEN, SSC .
STEROIDS, 1976, 28 (02) :179-196
[2]  
Battaglia DE, 1996, HUM REPROD, V11, P2217
[3]  
BATTAGLIA DE, 1997, MOL HUM REPROD, V2, P845
[4]  
Burger HG, 1998, CLIN ENDOCRINOL, V48, P809
[5]   INHIBIN, ACTIVIN, AND FOLLISTATIN - REGULATION OF FOLLICLE-STIMULATING-HORMONE MESSENGER-RIBONUCLEIC-ACID LEVELS [J].
CARROLL, RS ;
CORRIGAN, AZ ;
GHARIB, SD ;
VALE, W ;
CHIN, WW .
MOLECULAR ENDOCRINOLOGY, 1989, 3 (12) :1969-1976
[6]   INSULIN-LIKE GROWTH-FACTOR BINDING-PROTEIN PROFILES IN HUMAN OVARIAN FOLLICULAR-FLUID CORRELATE WITH FOLLICULAR FUNCTIONAL STATUS [J].
CATALDO, NA ;
GIUDICE, LC .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1992, 74 (04) :821-829
[7]   Interferon-γ and activin A promote insulin-like growth factor-binding protein-2 and -4 accumulation by human luteinizing granulosa cells, and interferon-γ promotes their apoptosis [J].
Cataldo, NA ;
Fujimoto, VY ;
Jaffe, RB .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (01) :179-186
[8]  
CHRISTMAN GM, 1991, FERTIL STERIL, V55, P1099
[9]   Hormonal regulation of apoptosis in early antral follicles: Follicle-stimulating hormone as a major survival factor [J].
Chun, SY ;
Eisenhauer, KM ;
Minami, S ;
Billig, H ;
Perlas, E ;
Hsueh, AJW .
ENDOCRINOLOGY, 1996, 137 (04) :1447-1456
[10]  
DIBLASIO AM, 1994, FERTIL STERIL, V61, P483