Construction of small RNA cDNA libraries for deep sequencing

被引：184

作者：

Lu, Cheng

Meyers, Blake C.

Green, Pamela J. ^{[1
]}

机构：

[1] Univ Delaware, Dept Plant & Soil Sci, Delaware Biotechnol Inst, Newark, DE 19711 USA

[2] Univ Delaware, Coll Marine & Earth Sci, Newark, DE 19711 USA

来源：

METHODS | 2007年 / 43卷 / 02期

基金：

美国国家科学基金会;

关键词：

small RNAs; miRNAs; siRNAs; high-throughput sequencing; 454;

D O I：

10.1016/j.ymeth.2007.05.002

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Small RNAs (21-24 nucleotides) including microRNAs (miRNAs) and small interfering RNAs (siRNAs) are potent regulators of gene expression in both plants and animals. Several hundred genes encoding miRNAs and thousands of siRNAs have been experimentally identified by cloning approaches. New sequencing technologies facilitate the identification of these molecules and provide global quantitative expression data in a given biological sample. Here, we describe the methods used in our laboratory to construct small RNA cDNA libraries for high-throughput sequencing using technologies such as MPSS, 454 or SBS. (C) 2007 Elsevier Inc. All rights reserved.

引用

收藏

页码：110 / 117

页数：8

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