Genome size, complexity, and ploidy of the pathogenic fungus Histoplasma capsulatum

被引:35
作者
Carr, J [1 ]
Shearer, G [1 ]
机构
[1] Univ So Mississippi, Dept Biol Sci, Ctr Mol & Cellular Biosci, Hattiesburg, MS 39406 USA
关键词
D O I
10.1128/JB.180.24.6697-6703.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The genome size, complexity, and ploidy of the dimorphic pathogenic fungus Histoplasma capsulatum was determined by using DNA renaturation kinetics, genomic reconstruction, and how cytometry, Nuclear DNA was isolated from two strains, G186AS and Downs, and analyzed by renaturation kinetics and genomic reconstruction with three putative single-copy genes (calmodulin, alpha-tubulin, and beta-tubulin), G186AS was found to have a genome of approximately 2.3 x 10(7) bp with less than 0.5% repetitive sequences. The Downs strain, however, was found to have a genome approximately 40% larger with more than 16 times more repetitive DNA. The Downs genome was determined to be 3.2 x 10(7) bp with approximately 8% repetitive DNA. To determine ploidy, the DNA mass per cell measured by flow cytometry was compared with the In genome estimate to yield a DNA index (DNA per cell/1n genome size). Strain G186AS was found to have a DNA index of 0.96, and Downs had a DNA index of 0.94, indicating that both strains are haploid. Genomic reconstruction and Southern blot data obtained with alpha- and beta-tubulin probes indicated that some genetic duplication has occurred in the Downs strain, which may be aneuploid or partially diploid.
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收藏
页码:6697 / 6703
页数:7
相关论文
共 30 条
[21]   IRREVERSIBLE BLOCK OF THE MYCELIAL-TO-YEAST PHASE-TRANSITION OF HISTOPLASMA-CAPSULATUM [J].
MEDOFF, G ;
SACCO, M ;
MARESCA, B ;
SCHLESSINGER, D ;
PAINTER, A ;
KOBAYASHI, GS ;
CARRATU, L .
SCIENCE, 1986, 231 (4737) :476-479
[22]   PROGRAM FOR LEAST-SQUARES ANALYSIS OF REASSOCIATION AND HYBRIDIZATION DATA [J].
PEARSON, WR ;
DAVIDSON, EH ;
BRITTEN, RJ .
NUCLEIC ACIDS RESEARCH, 1977, 4 (06) :1727-1737
[23]   The genome and genes of Neurospora crassa [J].
Radford, A ;
Parish, JH .
FUNGAL GENETICS AND BIOLOGY, 1997, 21 (03) :258-266
[24]   CLONING AND ANALYSIS OF CDNA-ENCODING AN ELONGATION-FACTOR 1-ALPHA FROM THE DIMORPHIC FUNGUS HISTOPLASMA-CAPSULATUM [J].
SHEARER, G .
GENE, 1995, 161 (01) :119-123
[25]   USE OF MITOCHONDRIAL AND RIBOSOMAL DNA POLYMORPHISMS TO CLASSIFY CLINICAL AND SOIL ISOLATES OF HISTOPLASMA-CAPSULATUM [J].
SPITZER, ED ;
LASKER, BA ;
TRAVIS, SJ ;
KOBAYASHI, GS ;
MEDOFF, G .
INFECTION AND IMMUNITY, 1989, 57 (05) :1409-1412
[26]   LOW REPETITIVE DNA CONTENT IN ASPERGILLUS-NIDULANS [J].
TIMBERLAKE, WE .
SCIENCE, 1978, 202 (4371) :973-975
[27]   CLASSIFICATION OF HISTOPLASMA-CAPSULATUM ISOLATES BY RESTRICTION FRAGMENT POLYMORPHISMS [J].
VINCENT, RD ;
GOEWERT, R ;
GOLDMAN, WE ;
KOBAYASHI, GS ;
LAMBOWITZ, AM ;
MEDOFF, G .
JOURNAL OF BACTERIOLOGY, 1986, 165 (03) :813-818
[28]   A SENSITIVE FLUOROMETRIC ASSAY FOR THE DETERMINATION OF DNA [J].
VYTASEK, R .
ANALYTICAL BIOCHEMISTRY, 1982, 120 (02) :243-248
[29]   DEVELOPMENT OF A GENETIC-TRANSFORMATION SYSTEM FOR HISTOPLASMA-CAPSULATUM - COMPLEMENTATION OF URACIL AUXOTROPHY [J].
WORSHAM, PL ;
GOLDMAN, WE .
MOLECULAR & GENERAL GENETICS, 1990, 221 (03) :358-362
[30]   CHROMOSOME-LENGTH POLYMORPHISM IN FUNGI [J].
ZOLAN, ME .
MICROBIOLOGICAL REVIEWS, 1995, 59 (04) :686-&