Use of different methods for testing antioxidative activity of oregano essential oil

被引:773
作者
Kulisic, T
Radonic, A
Katalinic, V
Milos, M
机构
[1] Univ Split, Fac Chem Technol, Dept Biochem & Food Chem, Split 21000, Croatia
[2] Univ Split, Fac Chem Technol, Dept Organ Chem, Split 21000, Croatia
[3] Univ Split, Inst Naval Med, Split 21000, Croatia
关键词
Origanum vulgare L; essential oil; natural antioxidants; antioxidant activity; beta-carotene bleaching; DPPH radical scavenging;
D O I
10.1016/j.foodchem.2003.07.024
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The antioxidant properties of the essential oil from oregano in relation to its chemical composition were examined. The antioxidant activity was investigated with three different methods: the beta-carotene bleaching (BCB) test, the 2,2'-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method and the thiobarbituric acid reactive species (TBARS) assay. It was found that the total essential oil, its fraction as well as its pure constituents have a significant antioxidant effect when tested by each method, respectively. Generally the antioxidant activity of the oregano essential oil is less effective than the ascorbic acid, but comparable with the alpha-tocopherol and the synthetic antioxidant butylated hydroxytoluene (BHT). The synergy among minor oxygen containing compounds was suggested as possible factor, which influenced the antioxidant power of the oregano essential oil. The antioxidant concentrations influenced its antioxidant power, too. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:633 / 640
页数:8
相关论文
共 41 条
[21]  
Pokorny J., 1991, Trends in Food Science & Technology, V2, P223, DOI 10.1016/0924-2244(91)90695-F
[22]   PARADOXICAL BEHAVIOR OF ANTIOXIDANTS IN FOOD AND BIOLOGICAL-SYSTEMS [J].
PORTER, WL .
TOXICOLOGY AND INDUSTRIAL HEALTH, 1993, 9 (1-2) :93-122
[23]  
Pratt D.E., 1980, Autoxidation in Food and Biological Systems, P283, DOI [10.1007/978-1-4757-9351-2_18, DOI 10.1007/978-1-4757-9351-2_18]
[24]   Antioxidant activity of selected essential oil components in two lipid model systems [J].
Ruberto, G ;
Baratta, MT .
FOOD CHEMISTRY, 2000, 69 (02) :167-174
[25]  
Shahidi F, 2000, NAHRUNG, V44, P158, DOI 10.1002/1521-3803(20000501)44:3&lt
[26]  
158::AID-FOOD158&gt
[27]  
3.0.CO
[28]  
2-L
[29]   PHENOLIC ANTIOXIDANTS [J].
SHAHIDI, F ;
JANITHA, PK ;
WANASUNDARA, PD .
CRITICAL REVIEWS IN FOOD SCIENCE AND NUTRITION, 1992, 32 (01) :67-103
[30]  
Tsimidou M., 1994, SPICES HERBS EDIBLE, P273