Bismuth Film Electrode as Sensing Platform for IgE-anti-IgE Interactions

被引：7

作者：

Anik, Ulku ^{[1
]}

Cevik, Serdar ^{[1
]}

Timur, Suna ^{[2
]}

机构：

[1] Mugla Univ, Fac Arts & Sci, Dept Chem, TR-48000 Kotekli, Mugla, Turkey

[2] Ege Univ, Fac Sci, Dept Biochem, TR-35100 Izmir, Turkey

来源：

ELECTROANALYSIS | 2011年 / 23卷 / 10期

关键词：

Bismuth film; Sensors; IgE; anti-IgE; ANODIC-STRIPPING VOLTAMMETRY; HEAVY-METALS; TRACE-METALS; MICROELECTRODE; TRANSDUCER; USAGE; LEAD;

D O I：

10.1002/elan.201100047

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this study, a bismuth film was used as a sensing platform for immunoreaction assay which includes the interactions between IgE and anti-IgE molecules. For preparing this assay, IgE was deposited on a carbon paste electrode via bismuth(III) cations without using any membrane or functional reagent. The immobilized reagents and interaction of anti-IgE on this formation were monitored by using atomic force microscopy technique and electrochemical impedance spectroscopy. Then some experimental parameters like the effect of IgE amount and the IgE anti-IgE interaction time were optimized. Under the optimal experimental conditions, the binding of anti-IgE was also examined by following current decrease in differential pulse voltammograms of neutral red.

引用

页码：2379 / 2385

页数：7

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