In vitro encystation and excystation of Blastocystis ratti

被引：17

作者：

Chen, XQ ^{[1
]}

Singh, M ^{[1
]}

Howe, J ^{[1
]}

Ho, LC ^{[1
]}

Tan, SW ^{[1
]}

Yap, EH ^{[1
]}

机构：

[1] Natl Univ Singapore, Dept Microbiol, Fac Med, Singapore 119260, Singapore

来源：

PARASITOLOGY | 1999年 / 118卷

关键词：

Blastocystis ratti; encystation; excystation;

D O I：

10.1017/S0031182098003667

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Cysts of Blastocystis ratti were produced in vitro by culturing the parasite in Iscove's modified Dulbecco's medium (IMDM) with increasing concentrations of horse serum. Yields up to 3 x 10(6) cysts/ml of culture medium were obtained after 72 h. Encystation efficiency was time, strain and inoculum size dependent. A viability of > 70 % was determined by flow cytometry employing fluorescein diacetate and propidium iodide staining. The presence of chitin as a cyst wall component was demonstrated by Calcofluor White M2R staining with which cystic stages showed blue fluorescence. The changes in morphology during excystation were examined by transmission electron microscopy. The cyst enlarged in size and some vacuoles appeared within the condensed cytoplasm. The vacuoles were full of inclusions and small glycogen aggregates. Coalescence of the vacuoles led to central body formation. Glycogen deposits were prominent throughout the excystation process. Some cysts divided by binary fission before the completion of the excystation.

引用

页码：151 / 160

页数：10

共 17 条

[1] IDENTIFICATION OF THE STRUCTURAL COMPONENT IN THE CYST WALL OF ENTAMOEBA-INVADENS [J].

ARROYOBEGOVICH, A ;

CARABEZTREJO, A ;

RUIZHERRERA, J .

JOURNAL OF PARASITOLOGY, 1980, 66 (05) :735-741

[2] BLASTOCYSTIS IN HUMANS AND ANIMALS - MORPHOLOGY, BIOLOGY, AND EPIZOOTIOLOGY [J].

BOREHAM, PFL ;

STENZEL, DJ .

ADVANCES IN PARASITOLOGY, VOL 32, 1993, 32 :1-70

[3]

Brumpt E., 1912, Bulletin de la Societe de Pathologie Exotique Paris, V5

[4]

BYERS TJ, 1991, REV INFECT DIS, V13, pS373

[5] Description of a Blastocystis species from Rattus norvegicus [J].

Chen, XQ ;

Singh, M ;

Ho, LC ;

Tan, SW ;

Ng, GC ;

Moe, KT ;

Yap, EH .

PARASITOLOGY RESEARCH, 1997, 83 (04) :313-318

[6]

Chen XQ, 1997, LAB ANIM SCI, V47, P91

[7] AXENIC CULTURE OF BLASTOCYSTIS-HOMINIS IN ISCOVE MODIFIED DULBECCO MEDIUM [J].

HO, LC ;

SINGH, M ;

SURESH, G ;

NG, GC ;

YAP, EH .

PARASITOLOGY RESEARCH, 1993, 79 (07) :614-616

[8] Observations on the ultrastructure and viability of the cystic stage of Blastocystis hominis from human feces [J].

Moe, KT ;

Singh, M ;

Howe, J ;

Ho, LC ;

Tan, SW ;

Ng, GC ;

Chen, XQ ;

Yap, EH .

PARASITOLOGY RESEARCH, 1996, 82 (05) :439-444

[9] Experimental Blastocystis hominis infection in laboratory mice [J].

Moe, KT ;

Singh, M ;

Howe, J ;

Ho, LC ;

Tan, SW ;

Chen, XQ ;

Ng, GC ;

Yap, EH .

PARASITOLOGY RESEARCH, 1997, 83 (04) :319-325

[10] MEMBRANE PROPERTIES OF LIVING MAMMALIAN CELLS AS STUDIED BY ENZYMATIC HYDROLYSIS OF FLUOROGENIC ESTERS [J].

ROTMAN, B ;

PAPERMASTER, BW .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1966, 55 (01) :134-+

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