Changes in vasoactive intestinal peptide and arginine vasopressin expression in the suprachiasmatic nucleus of the rat brain following footshock stress

被引:16
作者
Handa, Robert J. [1 ]
Zoeller, R. Thomas
McGivern, Robert F.
机构
[1] Colorado State Univ, Coll Vet Med, Div Neurosci, Dept Biomed Sci, Ft Collins, CO 80523 USA
[2] Univ Massachusetts, Dept Biol, Amherst, MA 01003 USA
[3] San Diego State Univ, Dept Psychol, San Diego, CA 92182 USA
关键词
stress; in situ hybridization; arginine vasopressin; vasoactive intestinal peptide; suprachiasmatic nucleus; paraventricular nucleus; supraoptic nucleus;
D O I
10.1016/j.neulet.2007.08.044
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
The neuropeptides, arginine vasopressin (AVP) and vasoactive intestinal polypeptide (VIP) are synthesized by neurons of the suprachiasmatic nucleus (SCN) of the hypothalamus and are important regulators of SCN function. Previous studies have demonstrated that acute exposure to stressors can disrupt circadian activity rhythms, suggesting the possibility of stress-related alterations in the expression of these neuropeptides within SCN neurons. In this study, we examined the effect of intermittent footshock stress on AVP mRNA and heterogeneous nuclear RNA (hnRNA) and VIP mRNA expression in neurons of the SCN. Young adult male Sprague/Dawley rats were subjected to 15 s of scrambled intermittent footshock (0.50 mA pulses, 1 pulse/s, 300 ms duration) every 5 min for 30 min. Animals were sacrificed 75 or 135 min after the onset of stress and brains examined for AVP mRNA and hnRNA, and VIP mRNA using in situ hybridization. Footshock stress increased AVP hnRNA levels at the 75 min time point whereas AVP mRNA was elevated at both the 75 and 135 min time points. In contrast, footshock stress decreased the number of cells expressing VIP mRNA in the SCN without changing hybridization level per cell. These data indicate that the disruptive effect of stress on activity rhythms correlate with alterations in the expression of regulatory peptides within the SCN. (c) 2007 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:99 / 104
页数:6
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