Investigation on the fluorescence quenching of 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) by certain estrogens and catechols

被引：14

作者：

Anbazhagan, V. ^{[1
]}

Kandavelu, V. ^{[1
]}

Kathiravan, A. ^{[1
]}

Renganathan, R. ^{[1
]}

机构：

[1] Bharathidasan Univ, Sch Chem, Tiruchirappalli 620024, India

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2008年 / 193卷 / 2-3期

关键词：

2.3-diazabicyclo[2.2.2]oct-2-ene; fluorescence quenching; estrogens; catechols;

D O I：

10.1016/j.jphotochem.2007.06.026

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The fluorescence quenching of singlet excited 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO), a fluorescent probe for antioxidants, by various estrogens and certain catechols has been investigated. The time resolved and steady state fluorescence quenching experiments were conducted in acetonitrile and dichloromethane. The bimolecular quenching rate constant (Q values are in the range of 10(7)-10(9) M-1 s(-1) for estrogens and 10(9)-10(10) M-1 s(-1) for catechols. In the case of estrogens, a direct hydrogen atom abstraction is proposed, while exciplex. induced quenching becomes competitive for catechols with electron donating (ED) substituents. The quenching mechanism was analysed on the basis of exciplex formation, deuterium isotopic effects and cyclicvoltammetric studies. Further, the invitro-antioxidant activity of estrogens and catechols were evaluated with rat liver catalase by gel electrophoresis. The data suggest the involvement of hydrogen atom transfer in the fluorescence quenching of DBO by estrogens and catechols. (C) 2007 Elsevier B.V. All rights reserved.

引用

页码：204 / 212

页数：9

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