Characterization of lipoxygenase-1 null mutants in barley

被引：29

作者：

Hirota, N

Kaneko, T

Kuroda, H

Kaneda, H

Takashio, M

Ito, K

Takeda, K

机构：

[1] Sapporo Breweries Ltd, Bioresources Res & Dev Labs, Gunma 3700393, Japan

[2] Sapporo Breweries Ltd, Frontier Labs Value Creat, Shizuoka 4250013, Japan

[3] Okayama Univ, Bioresources Res Inst, Okayama 7100046, Japan

来源：

THEORETICAL AND APPLIED GENETICS | 2005年 / 111卷 / 08期

关键词：

barley; lipoxygenase-1; null mutant; gene mapping; molecular marker;

D O I：

10.1007/s00122-005-0088-y

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

This study describes the discovery and characterization of lipoxygenase-1 (LOX-1) null mutants in barley. Six lines did not exhibit any significant LOX activity in the silenced seed extract. Immunological analysis showed that these lines lacked the authentic LOX-1 protein. Genetic analysis of the F-2 population revealed that this trait was governed by a single recessive gene located at the LoxA locus on chromosome 4H. The six LOX-1 null mutants shared similar features and the same unique polymorphism in a structural gene region, implying that these mutants might be derived from the same ancestral origin.

引用

页码：1580 / 1584

页数：5

共 28 条

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