Impact of Extracellular Nuclease Production on the Biofilm Phenotype of Staphylococcus aureus under In Vitro and In Vivo Conditions

被引:70
作者
Beenken, Karen E. [1 ]
Spencer, Horace [2 ]
Griffin, Linda M. [1 ]
Smeltzer, Mark S. [1 ]
机构
[1] Univ Arkansas Med Sci, Dept Microbiol & Immunol, Little Rock, AR 72205 USA
[2] Univ Arkansas Med Sci, Dept Biostat, Little Rock, AR 72205 USA
关键词
INFECTION; SARA; EXPRESSION; PROTEINS; RELEASE; MODEL; CIDA;
D O I
10.1128/IAI.06134-11
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Recent studies suggest that extracellular DNA promotes biofilm formation in Staphylococcus aureus and, conversely, that extracellular nucleases limit the ability to form a biofilm. S. aureus produces at least two extracellular nucleases, and in the study described in this report, we examined the impact of each of these nucleases on biofilm formation under both in vitro and in vivo conditions. Our results demonstrate that both nucleases impact biofilm formation in the clinical isolate UAMS-1. Under certain in vitro conditions, this impact is negative, with mutation of either or both of the nuclease genes (nuc1 and nuc2) resulting in an enhanced capacity to form a biofilm. However, this effect was not apparent in vivo in a murine model of catheter-associated biofilm formation. Rather, mutation of either or both nuclease genes appeared to limit biofilm formation to a degree that could be correlated with increased susceptibility to daptomycin.
引用
收藏
页码:1634 / 1638
页数:5
相关论文
共 23 条
[11]   Nuclease Modulates Biofilm Formation in Community-Associated Methicillin-Resistant Staphylococcus aureus [J].
Kiedrowski, Megan R. ;
Kavanaugh, Jeffrey S. ;
Malone, Cheryl L. ;
Mootz, Joe M. ;
Voyich, Jovanka M. ;
Smeltzer, Mark S. ;
Bayles, Kenneth W. ;
Horswill, Alexander R. .
PLOS ONE, 2011, 6 (11)
[12]   Mechanisms of biofilm formation in Staphylococcus epidermidis and Staphylococcus aureus:: functional molecules, regulatory circuits, and adaptive responses [J].
Mack, D ;
Becker, P ;
Chatterjee, I ;
Dobinsky, S ;
Knobloch, JKM ;
Peters, G ;
Rohde, H ;
Herrmann, M .
INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2004, 294 (2-3) :203-212
[13]   Modulation of eDNA Release and Degradation Affects Staphylococcus aureus Biofilm Maturation [J].
Mann, Ethan E. ;
Rice, Kelly C. ;
Boles, Blaise R. ;
Endres, Jennifer L. ;
Ranjit, Dev ;
Chandramohan, Lakshmi ;
Tsang, Laura H. ;
Smeltzer, Mark S. ;
Horswill, Alexander R. ;
Bayles, Kenneth W. .
PLOS ONE, 2009, 4 (06)
[14]   A novel Staphylococcus aureus biofilm phenotype mediated by the fibronectin-binding proteins, FnBPA and FnBPB [J].
O'Neill, Eoghan ;
Pozzi, Clarissa ;
Houston, Patrick ;
Humphreys, Hilary ;
Robinson, D. Ashley ;
Loughman, Anthony ;
Foster, Timothy J. ;
O'Gara, James P. .
JOURNAL OF BACTERIOLOGY, 2008, 190 (11) :3835-3850
[15]   A Novel Mechanism of Rapid Nuclear Neutrophil Extracellular Trap Formation in Response to Staphylococcus aureus [J].
Pilsczek, Florian H. ;
Salina, Davide ;
Poon, Karen K. H. ;
Fahey, Candace ;
Yipp, Bryan G. ;
Sibley, Christopher D. ;
Robbins, Stephen M. ;
Green, Francis H. Y. ;
Surette, Mike G. ;
Sugai, Motoyuki ;
Bowden, M. Gabriela ;
Hussain, Muzaffar ;
Zhang, Kunyan ;
Kubes, Paul .
JOURNAL OF IMMUNOLOGY, 2010, 185 (12) :7413-7425
[16]   Staphylococcus aureus CidA and LrgA Proteins Exhibit Holin-Like Properties [J].
Ranjit, Dev K. ;
Endres, Jennifer L. ;
Bayles, Kenneth W. .
JOURNAL OF BACTERIOLOGY, 2011, 193 (10) :2468-2476
[17]   Molecular control of bacterial death and lysis [J].
Rice, Kelly C. ;
Bayles, Kenneth W. .
MICROBIOLOGY AND MOLECULAR BIOLOGY REVIEWS, 2008, 72 (01) :85-+
[18]   The cidA murein hydrolase regulator contributes to DNA release and biofilm development in Staphylococcus aureus [J].
Rice, Kelly C. ;
Mann, Ethan E. ;
Endres, Jennifer L. ;
Weiss, Elizabeth C. ;
Cassat, James E. ;
Smeltzer, Mark S. ;
Bayles, Kenneth W. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (19) :8113-8118
[19]   Prevention and Treatment of Staphylococcus Biofilms [J].
Speziale, Pietro ;
Visai, Livia ;
Rindi, Simonetta ;
Pietrocola, Giampiero ;
Provenza, Giulio ;
Provenzano, Maria .
CURRENT MEDICINAL CHEMISTRY, 2008, 15 (30) :3185-3195
[20]   The staphylococcal nuclease prevents biofilm formation in Staphylococcus aureus and other biofilm-forming bacteria [J].
Tang JunNi ;
Kang MingSong ;
Chen HuanChun ;
Shi XianMing ;
Zhou Rui ;
Chen Juan ;
Du YiWu .
SCIENCE CHINA-LIFE SCIENCES, 2011, 54 (09) :863-869