In situ hybridization for mRNA detection in Arabidopsis tissue sections

被引：60

作者：

Brewer, Philip B. ^{[1
]}

Heisler, Marcus G.

Hejatko, Jan

Friml, Jiri

Benkova, Eva

机构：

[1] Univ Tubingen, Ctr Plant Mol Biol, D-72076 Tubingen, Germany

[2] Monash Univ, Sch Biol Sci, Clayton, Vic 3800, Australia

[3] Masaryk Univ, Dept Funct Genom & Proteom, Lab Mol Plant Physiol, CR-62500 Brno, Czech Republic

[4] CALTECH, Div Biol, Pasadena, CA 91125 USA

来源：

NATURE PROTOCOLS | 2006年 / 1卷 / 03期

关键词：

D O I：

10.1038/nprot.2006.226

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Plant biology is currently confronted with an overflow of expression profile data provided by high-throughput microarray transcription analyses. However, the tissue and cellular resolution of these techniques is limited. Thus, it is still necessary to examine the expression pattern of selected candidate genes at a cellular level. Here we present an in situ mRNA hybridization method that is routinely used in the analysis of gene expression patterns. The protocol is optimized for mRNA localizations in sectioned tissue of Arabidopsis seedlings including embryos, roots, hypocotyls, young primary leaves and flowers. The detailed protocol, recommended controls and troubleshooting are presented along with examples of application. The total time for the process is 10 days.

引用

页码：1462 / 1467

页数：6

共 5 条

[1]

Braissant O., 1998, BIOCHEMICA, V1, P10

[2] PETAL LOSS, a trihelix transcription factor gene, regulates perianth architecture in the Arabidopsis flower [J].

Brewer, PB ;

Howles, PA ;

Dorian, K ;

Griffith, ME ;

Ishida, T ;

Kaplan-Levy, RN ;

Kilinc, A ;

Smyth, DR .

DEVELOPMENT, 2004, 131 (16) :4035-4045

[3]

Heisler MGB, 2001, DEVELOPMENT, V128, P1089

[4] The putative sensor histidine kinase CKI1 is involved in female gametophyte development in Arabidopsis [J].

Hejátko, J ;

Pernisová, M ;

Eneva, T ;

Palme, K ;

Brzobohaty, B .

MOLECULAR GENETICS AND GENOMICS, 2003, 269 (04) :443-453

[5] A KNOTTED1-LIKE HOMEOBOX GENE IN ARABIDOPSIS IS EXPRESSED IN THE VEGETATIVE MERISTEM AND DRAMATICALLY ALTERS LEAF MORPHOLOGY WHEN OVEREXPRESSED IN TRANSGENIC PLANTS [J].

LINCOLN, C ;

LONG, J ;

YAMAGUCHI, J ;

SERIKAWA, K ;

HAKE, S .

PLANT CELL, 1994, 6 (12) :1859-1876

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