An RNA ligase essential for RNA editing and survival of the bloodstream form of Trypanosoma brucei

被引：214

作者：

Schnaufer, A

Panigrahi, AK

Panicucci, B

Igo, RP

Salavati, R

Stuart, K

机构：

[1] Seattle Biomed Res Inst, Seattle, WA 98109 USA

[2] Univ Washington, Dept Pathobiol, Seattle, WA 98195 USA

来源：

SCIENCE | 2001年 / 291卷 / 5511期

关键词：

D O I：

10.1126/science.1058655

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

RNA editing in trypanosomes occurs by a series of enzymatic steps that are catalyzed by a macromolecular complex. The TbMP52 protein is shown to be a component of this complex, to have RNA Ligase activity, and to be one of two adenylatable proteins in the complex. Regulated repression of TbMP52 blocks editing, which shows that it is a functional component of the editing complex. This repression is lethal in bloodforms of the parasite, indicating that editing is essential in the mammalian stage of the life cycle. The editing complex, which is present in all kinetoplastid parasites, may thus be a chemotherapeutic target.

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页码：2159 / 2162

页数：4

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