Genome analysis of a novel Shiga toxin 1 (Stx1)-converting phage which is closely related to Stx2-converting phages but not to other Stx1-converting phages

被引：33

作者：

Sato, T

Shimizu, T

Watarai, M

Kobayashi, M

Kano, S

Hamabata, T

Takeda, Y

Yamasaki, S

机构：

[1] Int Med Ctr Japan, Res Inst, Shinju Ku, Toyama, Japan

[2] Univ Tsukuba, Inst Basic Med Sci, Tsukuba, Ibaraki 3058575, Japan

[3] Natl Inst Infect Dis, Shinju Ku, Tokyo 1628640, Japan

[4] Osaka Prefecture Univ, Grad Sch Agr & Biol Sci, Sakai, Osaka 5918531, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 13期

关键词：

D O I：

10.1128/JB.185.13.3966-3971.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two Stx-converting phages, designated Stx1phi and Stx2phi-II, were isolated from an Escherichia coli O157:H7 strain, Morioka V526, and their entire nucleotide sequences were determined. The genomes of both phages were similar except for the stx gene-flanking regions. Comparing these phages to other known Stx-converting phages, we concluded that Stx1phi is a novel Stx1-converting phage closely related to Stx2-converting phages so far reported.

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页码：3966 / 3971

页数：6

相关论文

共 23 条

[1] Influence of RecA on in vivo virulence and Shiga toxin 2 production in Escherichia coli pathogens [J].

Fuchs, S ;

Mühldorfer, I ;

Donohue-Rolfe, A ;

Kerényi, M ;

Emödy, L ;

Alexiev, R ;

Nenkov, P ;

Hacker, J .

MICROBIAL PATHOGENESIS, 1999, 27 (01) :13-23

[2] Strain-specific differences in the amount of shiga toxin released from enterohemorrhagic Escherichia coli O157 following exposure to subinhibitory concentrations of antimicrobial agents [J].

Grif, K ;

Dierich, MP ;

Karch, H ;

Allerberger, F .

EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES, 1998, 17 (11) :761-766

[3] Shiga toxins even when different are encoded at identical positions in the genomes of related temperate bacteriophages [J].

Karch, H ;

Schmidt, H ;

Janetzki-Mittmann, C ;

Scheef, J ;

Kröger, M .

MOLECULAR AND GENERAL GENETICS, 1999, 262 (4-5) :600-607

[4] Toxin gene expression by shiga toxin-producing Escherichia coli:: the role of antibiotics and the bacterial SOS response [J].

Kimmitt, PT ;

Harwood, CR ;

Barer, MR .

EMERGING INFECTIOUS DISEASES, 2000, 6 (05) :458-465

[5]

Köhler B, 2000, MICROBIOL-UK, V146, P1085, DOI 10.1099/00221287-146-5-1085

[6] Identification of an insertion sequence, IS1203 variant, in a Shiga toxin 2 gene of Escherichia coli O157:H7 [J].

Kusumoto, M ;

Nishiya, Y ;

Kawamura, Y ;

Shinagawa, K .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 1999, 87 (01) :93-96

[7] Complete nucleotide sequence of the prophage VT2-Sakai carrying the verotoxin 2 genes of the enterohemorrhagic Escherichia coli O57:H7 derived from the Sakai outbreak [J].

Makino, K ;

Yokoyama, K ;

Kubota, Y ;

Yutsudo, CH ;

Kimura, S ;

Kurokawa, K ;

Ishii, K ;

Hattori, M ;

Tatsuno, I ;

Abe, H ;

Iida, T ;

Yamamoto, K ;

Onishi, M ;

Hayashi, T ;

Yasunaga, T ;

Honda, T ;

Sasakawa, C ;

Shinagawa, H .

GENES & GENETIC SYSTEMS, 1999, 74 (05) :227-239

[8] Spontaneous tandem amplification and deletion of the Shiga toxin operon in Shigella dysenteriae 1 [J].

McDonough, MA ;

Butterton, JR .

MOLECULAR MICROBIOLOGY, 1999, 34 (05) :1058-1069

[9]

Miyamoto H, 1999, DNA Res, V6, P235, DOI 10.1093/dnares/6.4.235

[10] Arrangement and functional identification of genes in the regulatory region of lambdoid phage H-19B, a carrier of a Shiga-like toxin [J].

Neely, MN ;

Friedman, DI .

GENE, 1998, 223 (1-2) :105-113