The role of TCR stimulation and. TGF-β in controlling the expression of CD94/NKG2A receptors on CD8 T cells

被引：32

作者：

Gunturi, A ^{[1
]}

Berg, RE ^{[1
]}

Crossley, E ^{[1
]}

Murray, S ^{[1
]}

Forman, J ^{[1
]}

机构：

[1] Univ Texas, SW Med Ctr, Ctr Immunol, Dallas, TX 75390 USA

来源：

EUROPEAN JOURNAL OF IMMUNOLOGY | 2005年 / 35卷 / 03期

关键词：

CD8 T cells; cytokines; T cell receptors; CD94;

D O I：

10.1002/eji.200425735

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Following antigen recognition, murine CD8 T cells express CD94/NKG2A receptors. Our results show that this up-regulation occurs rapidly in vitro and is accompanied by an similar to 8-fold increase in CD94 and similar to 125-fold increase in NKG2A mRNA. In contrast, only a twofold increase in NKG2C mRNA is noted. The addition of TGF-beta, but not IL-10, IL-12 or IL-15, leads to a further increase in cell membrane expression of these receptors, as well as a similar to 6-fold increase in mRNA for both chains. TGF-beta also increases CD94/NKG2A expression on memory CD8 T cells that are re-exposed to antigen. The effect of TGF-beta on increasing CD94/NKG2A expression on both naive and memory CD8 T cells occurs only when there is a concurrent stimulation through the TCR. In contrast, TGF-beta does not increase expression of CD94/NKG2A on resting or activated NK cells. We also show by using purified CD8 T cells, that TGF-beta acts directly on these cells. These results implicate a role for both antigen and TGF-beta in increasing expression of inhibitory CD94/ NKG2A receptors on CD8 T cells.

引用

页码：766 / 775

页数：10

共 46 条

[21]

Le Dréan E, 1998, EUR J IMMUNOL, V28, P264, DOI 10.1002/(SICI)1521-4141(199801)28:01<264::AID-IMMU264>3.0.CO

[22]

2-O

[23] Human CD94 gene expression: Dual promoters differing in responsiveness to IL-2 or IL-15 [J].

Lieto, LD ;

Borrego, F ;

You, CH ;

Coligan, JE .

JOURNAL OF IMMUNOLOGY, 2003, 171 (10) :5277-5286

[24] Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].

Livak, KJ ;

Schmittgen, TD .

METHODS, 2001, 25 (04) :402-408

[25]

Lohwasser S, 1999, EUR J IMMUNOL, V29, P755, DOI 10.1002/(SICI)1521-4141(199903)29:03<755::AID-IMMU755>3.3.CO

[26]

2-O

[27] Viral and bacterial infections induce expression of multiple NK cell receptors in responding CD8+ T cells [J].

McMahon, CW ;

Zajac, AJ ;

Jamieson, AM ;

Corral, L ;

Hammer, GE ;

Ahmed, R ;

Raulet, DH .

JOURNAL OF IMMUNOLOGY, 2002, 169 (03) :1444-1452

[28] SUPPRESSOR T-CELLS GENERATED BY ORAL TOLERIZATION TO MYELIN BASIC-PROTEIN SUPPRESS BOTH INVITRO AND INVIVO IMMUNE-RESPONSES BY THE RELEASE OF TRANSFORMING GROWTH-FACTOR-BETA AFTER ANTIGEN-SPECIFIC TRIGGERING [J].

MILLER, A ;

LIDER, O ;

ROBERTS, AB ;

SPORN, MB ;

WEINER, HL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (01) :421-425

[29] CD94/NKG2 expression does not inhibit cytotoxic function of lymphocytic choriomeningitis virus-specific CD8+ T cells [J].

Miller, JD ;

Peters, M ;

Oran, AE ;

Beresford, GW ;

Harrington, L ;

Boss, JM ;

Altman, JD .

JOURNAL OF IMMUNOLOGY, 2002, 169 (02) :693-701

[30] HLA class I-specific inhibitory receptors in human T lymphocytes:: Interleukin 15-induced expression of CD94/NKG2A in superantigen- or alloantigen-activated CD8+ T cells [J].

Mingari, MC ;

Ponte, M ;

Bertone, S ;

Schiavetti, F ;

Vitale, C ;

Bellomo, R ;

Moretta, A ;

Moretta, L .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (03) :1172-1177

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