Structures of spider toxins:: Hydroxyindole-3-acetylpolyamines and a new generalized structure of type-E compounds obtained from the venom of the Joro spider, Nephila clavata

被引：24

作者：

Hisada, M

Fujita, T

Naoki, H

Itagaki, Y

Irie, H

Miyashita, M

Nakajima, T

机构：

[1] Suntory Inst Bioorgan Res, Shimamoto, Osaka 618, Japan

[2] Nagasaki Univ, Fac Pharmaceut Sci, Nagasaki 852, Japan

[3] Hokkaido Univ, Grad Sch Sci, Div Chem, Sapporo, Hokkaido 060, Japan

来源：

TOXICON | 1998年 / 36卷 / 08期

关键词：

D O I：

10.1016/S0041-0101(98)00086-5

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Facile structure determination of acylpolyamines, glutamatergic nerve blocker obtained from the venom of the Joro spider (Nephila clavata) was carried out with the use of mu-column LC/MS and high energy collision induced dissociation (CID) mass spectrometry. 6-hydroxyindole-3-acetyl was proposed previously as a putative partial structure, for the acyl moiety of hydroxyindole-type polyamines (NPTX-1 similar to 6). The NMR data obtained for NPTX-6, NPTX-687 and hydroxyindole-3-acetic acid which was released by acid hydrolysis of Nephila clavata crude venom extracts proved that the lipophilic head is the 4-hydroxyindole-3-acetic acid. Various hydroxyindole-3-acetyl polyamines were found in N, clavata venom and characterized by mass spectrometry. As a result, type-E, a new class of generalized acylpolyamine structure was proposed in addition to the previously reported polyamine backbones type-A to -D. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1115 / 1125

页数：11

共 18 条

[1]

CHIBA T, 1994, CHEM PHARM BULL, V42, P1864, DOI 10.1248/cpb.42.1864

[2]

Chiba T, 1995, CHEM PHARM BULL, V43, P2177, DOI 10.1248/cpb.43.2177

[3]

Fujita T, 1997, RAPID COMMUN MASS SP, V11, P1115, DOI 10.1002/(SICI)1097-0231(19970630)11:10<1115::AID-RCM908>3.0.CO

[4]

2-D