Adhesion of Preosteoblasts and Fibroblasts onto Poly(pentafluorostyrene)-Based Glycopolymeric Films and their Biocompatibility

被引：28

作者：

Babiuch, Krzysztof ^{[1
]}

Becer, C. Remzi ^{[1
]}

Gottschaldt, Michael ^{[1
,3
]}

Delaney, Joseph T. ^{[1
,3
]}

Weisser, Juergen ^{[2
]}

Beer, Birgitt ^{[2
]}

Wyrwa, Ralf ^{[2
]}

Schnabelrauch, Matthias ^{[2
,3
]}

Schubert, Ulrich S. ^{[1
,3
]}

机构：

[1] Univ Jena, Lab Organ & Macromol Chem IOMC, D-07743 Jena, Germany

[2] INNOVENT eV, Biomat Dept, D-07745 Jena, Germany

[3] Dutch Polymer Inst, NL-5612 AB Eindhoven, Netherlands

来源：

MACROMOLECULAR BIOSCIENCE | 2011年 / 11卷 / 04期

关键词：

adhesion; click reaction; coatings; functionalization of polymers; glycopolymer; AMPHIPHILIC BLOCK-COPOLYMERS; FREE-RADICAL POLYMERIZATION; WELL-DEFINED GLYCOPOLYMER; MULTIVALENT LIGANDS; CLICK CHEMISTRY; CELL-ADHESION; SUGAR; DESIGN; POLYMERS; LECTIN;

D O I：

10.1002/mabi.201000374

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An efficient and metal-catalyst free method of glycopolymer synthesis via thiol/para-fluorine "click'' reaction was used to graft acetylated 1-thio-beta-D-glucopyranose and 1-thio-beta-D-galactopyranose onto a homopolymer of pentafluorostyrene (PFS) as well as onto a block copolymer of styrene and PFS. Subsequent deprotection of the carbohydrate moieties yielded well-defined, sugar-modified polymers (PDI < 1.2). The prepared polymers were not cytotoxic against 3T3 fibroblasts and MC3T3-E1 preosteoblasts. Furthermore, the water-insoluble copolymers were drop-cast and examined as synthetic biocompatible coatings on poly(propylene) substrates for culturing the investigated cell types. Both fibro- and preosteoblasts showed stable adhesion and proliferation on the glycopolymer-coated surfaces.

引用

页码：535 / 548

页数：14

共 83 条

[1] Osteoblast adhesion on biomaterials [J].

Anselme, K .

BIOMATERIALS, 2000, 21 (07) :667-681

[2] Click Chemistry beyond Metal-Catalyzed Cycloaddition [J].

Becer, C. Remzi ;

Hoogenboom, Richard ;

Schubert, Ulrich S. .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2009, 48 (27) :4900-4908

[3] Clicking Pentafluorostyrene Copolymers: Synthesis, Nanoprecipitation, and Glycosylation [J].

Becer, C. Remzi ;

Babiuch, Krzysztof ;

Pilz, David ;

Hornig, Stephanie ;

Heinze, Thomas ;

Gottschaldt, Michael ;

Schubert, Ulrich S. .

MACROMOLECULES, 2009, 42 (07) :2387-2394

[4] Fabrication and in vitro characterization of porous biodegradable composites based on phosphate glasses and oligolactide-containing polymer networks [J].

Brauer, Delia S. ;

Ruessel, Christian ;

Vogt, Sebastian ;

Weisser, Juergen ;

Schnabelrauch, Matthias .

JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A, 2007, 80A (02) :410-420

[5] A modular click approach to glycosylated polymeric beads:: Design, synthesis and preliminary lectin, recognition studies [J].

Chen, Gaojian ;

Tao, Lei ;

Mantovani, Giuseppe ;

Geng, J. ;

Nystrom, Daniel ;

Haddleton, David M. .

MACROMOLECULES, 2007, 40 (21) :7513-7520

[6] Synthesis of thiol-linked neoglycopolymers and thermo-responsive glycomicelles as potential drug carrier [J].

Chen, Gaojian ;

Amajjahe, Sadik ;

Stenzel, Martina H. .

CHEMICAL COMMUNICATIONS, 2009, (10) :1198-1200

[7] Fabricating Tissue Engineering Scaffolds for Simultaneous Cell Growth and Drug Delivery [J].

Chen, Wenhui ;

Tabata, Yasuhiko ;

Tong, Yen Wah .

CURRENT PHARMACEUTICAL DESIGN, 2010, 16 (21) :2388-2394

[8]

Chen YM, 2001, MACROMOL CHEM PHYSIC, V202, P3273, DOI 10.1002/1521-3935(20011101)202:17<3273::AID-MACP3273>3.0.CO

[9]

2-8

[10]

Chen YM, 2001, MACROMOL CHEM PHYSIC, V202, P3426, DOI 10.1002/1521-3935(20011101)202:17<3426::AID-MACP3426>3.0.CO

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