Characterization of conserved and novel microRNAs and their targets, including a TuMV-induced TIR-NBS-LRR class R gene-derived novel miRNA in Brassica

被引：164

作者：

He, Xiang-Feng ^{[1
,2
,3
]}

Fang, Yuan-Yuan ^{[1
,2
]}

Feng, Lei ^{[1
,2
,3
]}

Guo, Hui-Shan ^{[1
,2
]}

机构：

[1] Chinese Acad Sci, Inst Microbiol, State Key Lab Plant Genom, Beijing 100101, Peoples R China

[2] Chinese Acad Sci, Inst Microbiol, Natl Ctr Plant Gene Res Beijing, Beijing 100101, Peoples R China

[3] Chinese Acad Sci, Grad Sch, Beijing 100049, Peoples R China

来源：

FEBS LETTERS | 2008年 / 582卷 / 16期

基金：

中国国家自然科学基金;

关键词：

miRNA; TIR-NBS-LRR resistance gene;

D O I：

10.1016/j.febslet.2008.06.011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Nine conserved miRNA families and three potential novel miRNAs in Brassica rapa were identified from a small RNA library. The expression patterns of some conserved miRNAs had different tissue specificity in Brassica and Arabidopsis. One of the three potential miRNAs, named bra-miR1885, was verified as a true functional miRNA. It could be induced specifically by Turnip mosaic virus (TuMV) infection, and target TIR-NBS-LRR class disease-resistant transcripts for cleavage. Based on the hypothesis for de novo generation of new miRNA genes and the sequence similarity between bra-MIR1885 precursor loci and target transcript sequences, we suggest that bra-MIR1885 is a new miRNA gene that originated through inverted duplication events from TIR-NBS-LRR class disease-resistant protein-coding gene sequences, which became bra-miR1885 targets. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2445 / 2452

页数：8

共 30 条

[1] Cloning and characterization of micro-RNAs from moss [J].