The Rcs phosphorelay modulates the expression of plant cell wall degrading enzymes and virulence in Pectobacterium carotovorum ssp carotovorum

被引：18

作者：

Andresen, Liis ^{[1
]}

Koiv, Viia ^{[1
]}

Alamaee, Tiina ^{[1
]}

Maee, Andres ^{[1
]}

机构：

[1] Univ Tartu, Inst Mol & Cell Biol, Estonian Bioctr, Dept Genet, EE-51010 Tartu, Estonia

来源：

FEMS MICROBIOLOGY LETTERS | 2007年 / 273卷 / 02期

关键词：

phytopathogenic bacteria; two-component system; mutants; Rcs phosphorelay;

D O I：

10.1111/j.1574-6968.2007.00794.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Production of plant cell wall degrading enzymes, the major virulence factors of soft-rot Pectobacterium species, is controlled by many regulatory factors. Pectobacterium carotovorum ssp. carotovorum SCC3193 encodes an Rcs phosphorelay system that involves two sensor kinases, RcsC(Pcc), and RcsD(Pcc), and a response regulator RcsB(Pcc) as key components of this system, and an additional small lipoprotein RcsF(Pcc). This study indicates that inactivation of rcsC(Pcc), rcsD(Pcc) and rcsB(Pcc) enhances production of virulence factors with the highest effect detected for rcsB(Pcc). Interestingly, mutation of rcsF(Pcc), has no effect on virulence factors synthesis. These results suggest that in SCC3193 a parallel phosphorylation mechanism may activate the RcsB(Pcc) response regulator, which acts as a repressor suppressing the plant cell wall degrading enzyme production. Enhanced production of virulence factors in Rcs mutants is more pronounced when bacteria are growing in the absence of plant signal components.

引用

页码：229 / 238

页数：10

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