Third harmonic generation microscopy

被引:284
作者
Squier, JA [1 ]
Muller, M
Brakenhoff, GJ
Wilson, KR
机构
[1] Univ Calif San Diego, Dept Elect & Comp Engn, La Jolla, CA 92093 USA
[2] Univ Amsterdam, Dept Mol Cell Biol, Biocentrum Amsterdam, NL-1098 SM Amsterdam, Netherlands
[3] Univ Calif San Diego, Dept Chem, La Jolla, CA 92093 USA
来源
OPTICS EXPRESS | 1998年 / 3卷 / 09期
关键词
D O I
10.1364/OE.3.000315
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
Third harmonic generation microscopy is used to make dynamical images of living systems for the first time. A 100 fs excitation pulse at 1.2 mu m results in a 400 nm signal which is generated directly within the specimen. Chara plant rhizoids have been imaged, showing dynamic plant activity, and non-fading image characteristics even with continuous viewing, indicating prolonged viability under these THG-imaging conditions. (C) 1998 Optical Society of America.
引用
收藏
页码:315 / 324
页数:10
相关论文
共 8 条
[1]   Nonlinear scanning laser microscopy by third harmonic generation [J].
Barad, Y ;
Eisenberg, H ;
Horowitz, M ;
Silberberg, Y .
APPLIED PHYSICS LETTERS, 1997, 70 (08) :922-924
[2]  
Boyd R. W., 2020, Nonlinear Optics
[3]  
Müller M, 1998, J MICROSC-OXFORD, V191, P141, DOI 10.1046/j.1365-2818.1998.00357.x
[4]   3D microscopy of transparent objects using third-harmonic generation [J].
Müller, M ;
Squier, J ;
Wilson, KR ;
Brakenhoff, GJ .
JOURNAL OF MICROSCOPY, 1998, 191 :266-274
[5]  
Sievers A, 1996, TRENDS PLANT SCI, V1, P273
[6]  
SQUIER J, 1998, IN PRESS P ULTR PHEN
[7]   REFLECTED OPTICAL HARMONICS FROM DIELECTRIC MIRRORS [J].
TSANG, T .
APPLIED OPTICS, 1994, 33 (33) :7720-7724
[8]   OPTICAL 3RD-HARMONIC GENERATION AT INTERFACES [J].
TSANG, TYF .
PHYSICAL REVIEW A, 1995, 52 (05) :4116-4125