CXCR7 Protein Is Not Expressed on Human or Mouse Leukocytes

被引：66

作者：

Berahovich, Robert D. ^{[1
]}

Zabel, Brian A. ^{[1
]}

Penfold, Mark E. T. ^{[1
]}

Lewen, Susanna ^{[1
]}

Wang, Yu ^{[1
]}

Miao, Zhenhua ^{[1
]}

Gan, Lin ^{[2
]}

Pereda, Jaime ^{[4
]}

Dias, Jessica ^{[5
]}

Slukvin, Igor I. ^{[5
]}

McGrath, Kathleen E. ^{[3
]}

Jaen, Juan C. ^{[1
]}

Schall, Thomas J. ^{[1
]}

机构：

[1] ChemoCentryx Inc, Mountain View, CA 94043 USA

[2] Univ Rochester, Flaum Eye Inst, Rochester, NY 14642 USA

[3] Univ Rochester, Ctr Pediat Biomed Res, Rochester, NY 14642 USA

[4] Univ Santiago Chile, Human Embryol Unit, Santiago, Chile

[5] Univ Wisconsin, Madison, WI 53706 USA

来源：

JOURNAL OF IMMUNOLOGY | 2010年 / 185卷 / 09期

关键词：

ORPHAN RECEPTOR RDC1; CELL-MIGRATION; CHEMOKINE RECEPTORS; T-LYMPHOCYTES; CD34(+) CELLS; STEM-CELLS; LIGAND; ACTIVATION; SURVIVAL; GROWTH;

D O I：

10.4049/jimmunol.1001660

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Since the discovery that CXCR7 binds to CXCL12/SDF-1 alpha, the role of CXCR7 in CXCL12-mediated biological processes has been under intensive scrutiny. However, there is no consensus in the literature on the expression of CXCR7 protein by peripheral blood cells. In this study we analyzed human and mouse leukocytes and erythrocytes for CXCR7 protein expression, using a competitive CXCL12 binding assay as well as by flow cytometry and immunohistochemistry using multiple CXCR7 Abs. CXCR7(-/-) mice were used as negative controls. Together, these methods indicate that CXCR7 protein is not expressed by human peripheral blood T cells, B cells, NK cells, or monocytes, or by mouse peripheral blood leukocytes. CXCR7 protein is, however, expressed on mouse primitive erythroid cells, which supply oxygen to the embryo during early stages of development. These studies therefore suggest that, whereas CXCR7 protein is expressed by primitive RBCs during murine embryonic development, in adult mammals CXCR7 protein is not expressed by normal peripheral blood cells. The Journal of Immunology, 2010, 185: 5130-5139.

引用

页码：5130 / 5139

页数：10

共 31 条

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