Composition, uniqueness and variability of the epiphytic bacterial community of the green alga Ulva australis

被引:298
作者
Burke, Catherine [1 ]
Thomas, Torsten [1 ]
Lewis, Matt [2 ]
Steinberg, Peter [3 ,4 ]
Kjelleberg, Staffan [1 ,5 ]
机构
[1] Univ New S Wales, Ctr Marine Bioinnovat, Sch Biotechnol & Biomol Sci, Sydney, NSW 2052, Australia
[2] J Craig Venter Inst, Rockville, MD USA
[3] Univ New S Wales, Ctr Marine Bioinnovat, Sch Biol Earth & Environm Sci, Sydney, NSW 2052, Australia
[4] Sydney Inst Marine Sci, Sydney, NSW, Australia
[5] Nanyang Technol Univ, Sch Biol Sci, Singapore, Singapore
基金
澳大利亚研究理事会;
关键词
16S rRNA gene libraries; epiphytic bacterial community; macroalga; Ulva australis; lottery hypothesis; URCHIN HELIOCIDARIS-ERYTHROGRAMMA; MICROBIAL COMMUNITIES; PHYLOGENETIC ANALYSIS; FUNCTIONAL REDUNDANCY; NURSERY HABITATS; DEEP-SEA; DIVERSITY; ABUNDANCE; PATTERNS; SURFACE;
D O I
10.1038/ismej.2010.164
中图分类号
Q14 [生态学(生物生态学)];
学科分类号
071012 ; 0713 ;
摘要
Green Ulvacean marine macroalgae are distributed worldwide in coastal tidal and subtidal ecosystems. As for many living surfaces in the marine environment, little is known concerning the epiphytic bacterial biofilm communities that inhabit algal surfaces. This study reports on the largest published libraries of near full-length 16S rRNA genes from a marine algal surface (5293 sequences from six samples) allowing for an in-depth assessment of the diversity and phylogenetic profile of the bacterial community on a green Ulvacean alga. Large 16S rRNA gene libraries of surrounding seawater were also used to determine the uniqueness of this bacterial community. The surface of Ulva australis is dominated by sequences of Alphaproteobacteria and the Bacteroidetes, especially within the Rhodobacteriaceae, Sphingomonadaceae, Flavobacteriaceae and Sapropiraceae families. Seawater libraries were also dominated by Alphaproteobacteria and Bacteroidetes sequences, but were shown to be clearly distinct from U. australis libraries through the clustering of sequences into operational taxonomic units and Bray-Curtis similarity analysis. Almost no similarity was observed between these two environments at the species level, and only minor similarity was observed at levels of sequence clustering representing clades of bacteria within family and genus taxonomic groups. Variability between libraries of U. australis was relatively high, and a consistent sub-population of bacterial species was not detected. The competitive lottery model, originally derived to explain diversity in coral reef fishes, may explain the pattern of colonization of this algal surface. The ISME Journal (2011) 5, 590-600; doi:10.1038/ismej.2010.164; published online 4 November 2010
引用
收藏
页码:590 / 600
页数:11
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