Kinetics of hepatitis B surface antigen-specific immune responses in acute and chronic hepatitis B or after HBs vaccination:: Stimulation of the in vitro antibody response by interferon gamma

被引:64
作者
Böcher, WO [1 ]
Herzog-Hauff, S [1 ]
Schlaak, J [1 ]
zum Büschenfelde, KHM [1 ]
Löhr, HF [1 ]
机构
[1] Univ Mainz, Dept Immunol, D-6500 Mainz, Germany
关键词
D O I
10.1002/hep.510290120
中图分类号
R57 [消化系及腹部疾病];
学科分类号
摘要
Because cellular and humoral immune responses against the hepatitis B virus (HBV) surface antigen (HBs) might be crucial to overcome HBV infection, HBs-specific B- and T-cell responses of HBV patients and HBs vaccine recipients were analyzed quantitatively and functionally. In patients with acute hepatitis B (AHB), transient high anti-HBs-secreting B-cell frequencies were observed early after clinical onset, whereas 1 patient who probably developed chronic infection and chronic HBV carriers had absent or weak B- and T-cell responses. In HBs vaccine recipients, maximal HBs-specific B- and T-cell responses were detected after the first injection that decreased gradually before anti-HBs antibodies appeared in serum. Years after vaccination, anti-HBs-secreting B cells were enriched in the bone marrow. After in vitro stimulation with HBsAg, peripheral blood mononuclear cells (PBMC) of only 1 of 5 acute and 1 of 6 chronic HBV patients, but of all 6 vaccine recipients, secreted varying amounts of interferon gamma (IFN-gamma), but no interleukin-4 (IL-4) or IL-5. Furthermore, the addition of IFN-gamma, but not of IL-2, -4, -12, or IFN-alpha, resulted in strong increases of anti-HBs-secreting B cells in vaccine recipients and chronic carriers. In conclusion, circulating anti-HBs-secreting B cells were significantly higher in early acute hepatitis B or early after HBs vaccination than in chronic hepatitis B and decreased in the follow-up as a result of compartmentalization to lymphoid tissues. Release of IFN-gamma by antigen-stimulated T cells might be critical for anti-HBs formation.
引用
收藏
页码:238 / 244
页数:7
相关论文
共 36 条
  • [31] SYLVAN SPE, 1989, CLIN EXP IMMUNOL, V78, P207
  • [32] HEPATITIS-B VACCINE - DEMONSTRATION OF EFFICACY IN A CONTROLLED CLINICAL-TRIAL IN A HIGH-RISK POPULATION IN THE UNITED-STATES
    SZMUNESS, W
    STEVENS, CE
    HARLEY, EJ
    ZANG, EA
    OLESZKO, WR
    WILLIAM, DC
    SADOVSKY, R
    MORRISON, JM
    KELLNER, A
    [J]. NEW ENGLAND JOURNAL OF MEDICINE, 1980, 303 (15) : 833 - 841
  • [33] ACUTE EXACERBATIONS OF CHRONIC TYPE B-HEPATITIS ARE ACCOMPANIED BY INCREASED T-CELL RESPONSES TO HEPATITIS-B CORE AND E-ANTIGENS - IMPLICATIONS FOR HEPATITIS-B E-ANTIGEN SEROCONVERSION
    TSAI, SL
    CHEN, PJ
    LAI, MY
    YANG, PM
    SUNG, JL
    HUANG, JH
    HWANG, LH
    CHANG, TH
    CHEN, DS
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1992, 89 (01) : 87 - 96
  • [34] THERE IS NO CORRELATION BETWEEN FUNCTION AND LYMPHOKINE PRODUCTION OF HBS-ANTIGEN-SPECIFIC HUMAN CD4+-CLONED T-CELLS
    TSUTSUI, H
    MIZOGUCHI, Y
    MORISAWA, S
    [J]. SCANDINAVIAN JOURNAL OF IMMUNOLOGY, 1991, 34 (04) : 433 - 444
  • [35] VENTO S, 1987, LANCET, V2, P119
  • [36] DURATION OF IMMUNOGENICITY AND EFFICACY OF HEPATITIS-B VACCINE IN A YUPIK ESKIMO POPULATION
    WAINWRIGHT, RB
    MCMAHON, BJ
    BULKOW, LR
    HALL, DB
    FITZGERALD, MA
    HARPSTER, AP
    HADLER, SC
    LANIER, AP
    HEYWARD, WL
    [J]. JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1989, 261 (16): : 2362 - 2366