Differences in metabolite profile between blood plasma and serum

被引：161

作者：

Liu, Linsheng ^{[1
]}

Aa, Jiye ^{[1
]}

Wang, Guangji ^{[1
]}

Yan, Bei ^{[1
]}

Zhang, Ying ^{[1
]}

Wang, Xinwen ^{[1
]}

Zhao, Chunyan ^{[1
]}

Cao, Bei ^{[1
]}

Shi, Jian ^{[1
]}

Li, Mengjie ^{[1
]}

Zheng, Tian ^{[1
]}

Zheng, Yuanting ^{[1
]}

Hao, Gang ^{[1
]}

Zhou, Fang ^{[1
]}

Sun, Jianguo ^{[1
]}

Wu, Zimei ^{[2
]}

机构：

[1] China Pharmaceut Univ, Lab Metabol, Key Lab Drug Metab & Pharmacokinet, Nanjing 21009, Peoples R China

[2] Univ Auckland, Sch Pharm, Auckland 1142, New Zealand

来源：

ANALYTICAL BIOCHEMISTRY | 2010年 / 406卷 / 02期

关键词：

Plasma; Serum; Metabolomics; Gas chromatography/time-of-flight mass spectrometry; Principal components analysis; HEPARINIZED PLASMA; EXTRACTION; SAMPLES; TESTS;

D O I：

10.1016/j.ab.2010.07.015

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

In metabolomic research, blood plasma and serum have been considered to possess similar compositions and properties. Their perceived equivalence has resulted in researchers choosing arbitrarily between serum and plasma for analysis. Here, routine serum and plasma were prepared and their low-molecular-weight compounds were determined using gas chromatography/time-of-flight mass spectrometry. Principal components analysis was applied to process the acquired data, and marked differences in metabolite profiles were observed between serum and plasma. Of the 72 identified compounds, 36 (50%) discriminate serum from plasma, with 29 and 7 metabolites showing a significantly higher abundance (t test, P < 0.05) in serum and plasma, respectively. Incubation of blood had distinct effects on the analyte peak areas, with the effects being more pronounced for plasma than for serum and more pronounced for a shorter incubation than for a longer incubation. These results highlight the importance in choosing serum or plasma as the analytical sample and in stipulating the incubation time. Because incubation affected the analyte peak areas less in serum than in plasma, we recommend serum as the sample of choice in metabolomic studies. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：105 / 112

页数：8

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