Identification and characterization of a novel miR159 target not related to MYB in tomato

被引:44
作者
Buxdorf, Kobi [1 ]
Hendelman, Anat [1 ]
Stav, Ran [1 ]
Lapidot, Moshe [1 ]
Ori, Naomi [2 ]
Arazi, Tzahi [1 ]
机构
[1] Agr Res Org, Volcani Ctr, Inst Plant Sci, IL-50250 Bet Dagan, Israel
[2] Hebrew Univ Jerusalem, Fac Agr Food & Environm, Robert H Smith Inst Plant Sci & Genet Agr, IL-76100 Rehovot, Israel
关键词
Flower; Development; Leaf; MiRNA; MiR159; Tomato; ARABIDOPSIS-THALIANA; EARLY SPOROGENESIS; PATTERN-FORMATION; SEED-GERMINATION; PLANT MICRORNAS; ALEURONE CELLS; SMALL RNAS; GENE; EXPRESSION; PROTEIN;
D O I
10.1007/s00425-010-1231-9
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
MicroRNA 159 (miR159) is a highly conserved miRNA with roles in flowering under short days, anther development and seed germination via repression of GAMYB-like genes. In tomato, the function of miR159 (Sl-miR159) is currently unknown and target transcripts have not been experimentally validated. Here, we identified and characterized a new miR159 target gene (SGN-U567133) in Solanum lycopersicum (tomato) that is not related to MYB. SGN-U567133 is predominantly expressed in flowers and encodes a nuclear-localized protein that contains a unique NOZZLE-like domain at its N terminus. In tomato, SGN-U567133 represents a small gene family and orthologs have been identified in other plant species, all containing a conserved miR159 target site in their coding sequence. Accordingly, 5'-RACE cleavage assay supported miRNA-mediated cleavage of SGN-U567133 transcripts in vivo. Moreover, the SGN-U567133 transcript accumulated in P19-HA-expressing tomato leaves in which miRNA-mediated cleavage is inhibited. In addition, transgenic tomato plants expressing a miR159-resistant form of SGN-U567133 accumulated higher levels of the SGN-U567133 transcript and exhibited defects in leaf and flower development. Together, our results suggest that SGN-U567133 represents a novel class of miR159 targets in plants and raise the possibility that its post-transcriptional regulation by Sl-miR159 is essential for normal tomato development.
引用
收藏
页码:1009 / 1022
页数:14
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