Spz1, a novel bHLH-Zip protein, is specifically expressed in testis

被引：38

作者：

Hsu, SH

Shyu, HW

Hsieh-Li, HM

Li, H ^{[1
]}

机构：

[1] Acad Sinica, Inst Mol Biol, Taipei 11529, Taiwan

[2] Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan

[3] Taipei Med Coll, Inst Med Sci, Taipei, Taiwan

来源：

MECHANISMS OF DEVELOPMENT | 2001年 / 100卷 / 02期

关键词：

basic helix-loop-helix; leucine zipper; mouse; spermatogenesis; testis;

D O I：

10.1016/S0925-4773(00)00513-X

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We isolated a novel bHLH-Zip gene designated Spz1 from a mouse testis cDNA library. Spz1 is expressed specifically in the testis and epididymis. Immunofluorescence staining detected Spz1 protein in the nuclei of LFG6 Leydig cells. The ability of Spz1 protein to bind to the bHLH consensus-binding site, the E-box, was confirmed by EMSA, and a 9-bp asymmetric target site was identified by random selection and PCR amplification. Hormonal regulation of Spz1 was investigated and downregulation of Spz1 expression by testosterone and retinoic acid was found. This nuclear transcription factor may play a crucial role in spermatogenesis by regulating cell proliferation or differentiation through binding to specific DNA sequences like other bHLH-Zip molecules. (C) 2001 Elsevier Science Ireland Ltd. Ail rights reserved.

引用

页码：177 / 187

页数：11

共 64 条

[1] LOCALIZATION OF IGF-I IN ADULT-RATS - IMMUNOHISTOCHEMICAL STUDIES [J].