Monitoring cellular responses of engine-emitted particles by using a direct air-cell interface deposition technique

被引:35
作者
Cheng, MD
Malone, B
Storey, JME
机构
[1] Oak Ridge Natl Lab, Div Environm Sci, Oak Ridge, TN 37831 USA
[2] Oak Ridge Natl Lab, Engn Technol & Sci Div, Oak Ridge, TN 37831 USA
关键词
aerosol; aerosol deposition; air-cell interface; human lung cells; internal combustion engines; cytokines;
D O I
10.1016/S0045-6535(03)00555-1
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
The impacts of ultrafine airborne particles generated by diesel or gasoline engines on human lung cells have been investigated using a new in vitro cellular exposure technique. This technique enables direct deposition of the gasoline engine exhaust particles (GEP) and diesel engine exhaust particles (DEP) on human lung cells located at the air-cell interface on a transwell membrane in an exposure apparatus. The cellular responses to particle exposure were measured by the levels of IL-8 chemokines produced as a function of exposure time. The findings suggest that GEP and high-sulfur DEP induced the production of similar levels of IL-8 by unprimed A549 cells. The level of IL-8 produced by unprimed A549 cells in response to low-sulfur DEP was found lower than that produced in response to high-sulfur DEP and GEP. When cells were primed, simulating predisposed conditions, significant levels of IL-8 were produced. GEP triggered a much higher level of IL-8 production than DEP did. Furthermore, the time profile of IL-8 production induced by GEP was markedly different from that induced by DEP. The findings indicate that GEP could induce the production of higher levels of chemokines (i.e., IL-8) than DEP did, implying that exposure to GEP could be a greater health risk than exposure to DEP. (C) 2003 Elsevier Ltd. All rights reserved.
引用
收藏
页码:237 / 243
页数:7
相关论文
共 26 条
[1]   Diesel exhaust (DE)-induced cytokine expression in human bronchial epithelial cells -: A study with a new cell exposure system to freshly generated DE in vitro [J].
Abe, S ;
Takizawa, H ;
Sugawara, I ;
Kudoh, S .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2000, 22 (03) :296-303
[2]   The cytotoxic effects of diesel exhaust particles on human pulmonary artery endothelial cells in vitro: Role of active oxygen species [J].
Bai, YS ;
Suzuki, AK ;
Sagai, M .
FREE RADICAL BIOLOGY AND MEDICINE, 2001, 30 (05) :555-562
[3]   Diesel exhaust particles are taken up by human airway epithelial cells in vitro and alter cytokine production [J].
Boland, S ;
Baeza-Squiban, A ;
Fournier, T ;
Houcine, O ;
Gendron, MC ;
Chévrier, M ;
Jouvenot, G ;
Coste, A ;
Aubier, M ;
Marano, F .
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY, 1999, 276 (04) :L604-L613
[4]   Activation of transcription factors by diesel exhaust particles in human bronchial epithelial cells in vitro [J].
Bonvallot, V ;
Baeza-Squiban, A ;
Boland, S ;
Marano, F .
INHALATION TOXICOLOGY, 2000, 12 :359-364
[5]  
Carero ADP, 2001, ENVIRON MOL MUTAGEN, V37, P155
[6]   Impacts of venturi turbulent mixing on the size distributions of sodium chloride and dioctyl-phthalate aerosols [J].
Cheng, MD ;
Storey, JM ;
Wainman, T ;
Dam, T .
JOURNAL OF AEROSOL SCIENCE, 2002, 33 (03) :491-502
[7]  
Hiura TS, 1999, J IMMUNOL, V163, P5582
[8]   Peroxynitrite formation by diesel exhaust particles in alveolar cells: Links to pulmonary inflammation [J].
Ito, T ;
Ikeda, M ;
Yamasaki, H ;
Sagai, M ;
Tomita, T .
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 2000, 9 (1-2) :1-8
[9]   Benzene-extracted components are important for the major activity of diesel exhaust particles - Effect on interleukin-8 gene expression in human bronchial epithelial cells [J].
Kawasaki, S ;
Takizawa, H ;
Takami, K ;
Desaki, M ;
Okazaki, H ;
Kasama, T ;
Kobayashi, K ;
Yamamoto, K ;
Nakahara, K ;
Tanaka, M ;
Sagai, M ;
Ohtoshi, T .
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY, 2001, 24 (04) :419-426
[10]   Exposure of human lung cells to native diesel motor exhaust - development of an optimized in vitro test strategy [J].
Knebel, JW ;
Ritter, D ;
Aufderheide, M .
TOXICOLOGY IN VITRO, 2002, 16 (02) :185-192