Genetic Suppressor Screens in Haploids

被引：2

作者：

Bai, Xiaoying ^{[1
,2
,3
]}

Yang, Zhongan ^{[4
]}

Jiang, Hong ^{[4
]}

Lin, Shuo ^{[4
]}

Zon, Leonard I. ^{[1
,2
,3
]}

机构：

[1] Howard Hughes Med Inst, Boston, MA 02115 USA

[2] Childrens Hosp, Stem Cell Program, Boston, MA 02115 USA

[3] Harvard Univ, Div Hematol Oncol, Childrens Hosp Boston, Harvard Stem Cell Inst,Med Sch, Boston, MA USA

[4] Univ Calif Los Angeles, Dept Mol Cell & Dev Biol, Los Angeles, CA USA

来源：

ZEBRAFISH: GENETICS, GENOMICS AND INFORMATICS, 3RD EDITION | 2011年 / 104卷

关键词：

ZEBRAFISH; DESIGN; ART;

D O I：

10.1016/B978-0-12-374814-0.00007-0

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

As a vertebrate genetic model, the zebrafish has been well recognized for its strength in studying a variety of biological processes and human diseases. Traditional forward genetic screens in zebrafish have generated a large pool of mutants with interesting phenotypes resembling human diseases but the underlying mechanisms are not well understood. A powerful approach to elucidate the mechanisms of these mutants is the modifier screen, which identifies 2(nd)-site mutations that specifically enhance or block the phenotype of a given mutant. Here we described the first genetic suppressor screen in zebrafish, which identifies a novel transcriptional mechanism regulating erythropoiesis. In combination with the haploid genetics in zebrafish, we have shown the feasibility and strength of a modifier screen in zebrafish. This strategy will greatly broaden the utility of the zebrafish as a model for making original discoveries and establishing novel paradigms for understanding vertebrate biology.

引用

页码：129 / 136

页数：8

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