Analysis of the isoform of Xenopus euakryotic translation initiation factor 4E

被引:11
作者
Wakiyama, M [1 ]
Suzuki, A [1 ]
Saigoh, M [1 ]
Sakai, N [1 ]
Miyoshi, H [1 ]
Kojima, S [1 ]
Miura, K [1 ]
机构
[1] Gakushuin Univ, Inst Biomol Sci, Toshima Ku, Tokyo 1718588, Japan
关键词
translation initiation factor 4E; isoform; Xenopus;
D O I
10.1271/bbb.65.232
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We have found two isoforms of the eukaryotic translation initiation factor 4E (eIF4E) in Xenopus laevis. These proteins differ in length by 18 amino acids. Overexpression of either of the two eIF4E proteins modestly increase translation in Xenopus oocytes. The results suggest that both of these two isoforms function in translation.
引用
收藏
页码:232 / 235
页数:4
相关论文
共 14 条
[1]   OVEREXPRESSION OF EUKARYOTIC PROTEIN-SYNTHESIS INITIATION FACTOR-4E IN HELA-CELLS RESULTS IN ABERRANT GROWTH AND MORPHOLOGY [J].
DEBENEDETTI, A ;
RHOADS, RE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1990, 87 (21) :8212-8216
[2]  
DUNCAN R, 1987, J BIOL CHEM, V262, P380
[3]   BLOCKED STRUCTURE AT 5' TERMINUS OF MESSENGER-RNA FROM CYTOPLASMIC POLYHEDROSIS-VIRUS [J].
FURUICHI, Y ;
MIURA, KI .
NATURE, 1975, 253 (5490) :374-375
[4]   eIF4 initiation factors: Effectors of mRNA recruitment to ribosomes and regulators of translation [J].
Gingras, AC ;
Raught, B ;
Sonenberg, N .
ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :913-963
[5]  
HIREMATH LS, 1985, J BIOL CHEM, V260, P7843
[6]   Multiple isoforms of eukaryotic protein synthesis initiation factor 4E in Caenorhabditis elegans can distinguish between mono- and trimethylated mRNA cap structures [J].
Jankowska-Anyszka, M ;
Lamphear, BJ ;
Aamodt, EJ ;
Harrington, T ;
Darzynkiewicz, E ;
Stolarski, R ;
Rhoads, RE .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1998, 273 (17) :10538-10542
[7]  
KRIEG PA, 1987, METHOD ENZYMOL, V155, P397
[8]   Alternatively spliced transcripts from the Drosophila eIF4E gene produce two different cap-binding proteins [J].
Lavoie, CA ;
Lachance, PED ;
Sonenberg, N ;
Lasko, P .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (27) :16393-16398
[9]   MALIGNANT TRANSFORMATION BY A EUKARYOTIC INITIATION-FACTOR SUBUNIT THAT BINDS TO MESSENGER-RNA 5' CAP [J].
LAZARISKARATZAS, A ;
MONTINE, KS ;
SONENBERG, N .
NATURE, 1990, 345 (6275) :544-547
[10]   Binding analysis of Xenopus laevis translation initiation factor 4E (eIF4E) in initiation complex formation [J].
Miyoshi, H ;
Youtani, T ;
Ide, H ;
Hori, H ;
Okamoto, K ;
Ishikawa, M ;
Wakiyama, M ;
Nishino, T ;
Ishida, T ;
Miura, K .
JOURNAL OF BIOCHEMISTRY, 1999, 126 (05) :897-904