MALIGNANT TRANSFORMATION BY A EUKARYOTIC INITIATION-FACTOR SUBUNIT THAT BINDS TO MESSENGER-RNA 5' CAP

被引：849

作者：

LAZARISKARATZAS, A ^{[1
]}

MONTINE, KS ^{[1
]}

SONENBERG, N ^{[1
]}

机构：

[1] MCGILL UNIV,MCGILL CANC CTR,MONTREAL H3G 1Y6,QUEBEC,CANADA

来源：

NATURE | 1990年 / 345卷 / 6275期

关键词：

D O I：

10.1038/345544a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

EUKARYOTIC cellular mRNAs have a 5' cap structure (m7 GpppX) that facilitates binding to ribosomes and is required for efficient translation1-3. A specific initiation factor, eIF-4F, mediates the function of the cap4-6 and consists of three subunits7-10, one of which, eIF-4E, binds the cap. This subunit is present in limiting amounts in the cell11-13, and is thought to be regulated by phosphorylation12-16: decreased phosphorylation of eIF-4E following various treatments correlates with a decrease in cellular translation rate. These observations suggest that eIF-4E lies on the mitogenic signal transduction pathway, and we reasoned that overexpression of eIF-4E might profoundly affect cellular growth properties. We report here that overexpression of eIF-4E in NIH 3T3 and Rat 2 fibroblasts causes their tumorigenic transformation as determined by three criteria: formation of transformed foci on a monolayer of cells; anchorage-independent growth; and tumour formation in nude mice. © 1990 Nature Publishing Group.

引用

页码：544 / 547

页数：4

共 32 条

[1]

ABRAMSON RD, 1987, J BIOL CHEM, V262, P3826

[2] ALTERED MESSENGER-RNA CAP RECOGNITION ACTIVITY OF INITIATION FACTOR-4E IN THE YEAST-CELL CYCLE DIVISION MUTANT CDC33 [J].

ALTMANN, M ;

TRACHSEL, H .

NUCLEIC ACIDS RESEARCH, 1989, 17 (15) :5923-5931

[3]

ALTMANN M, 1989, J BIOL CHEM, V264, P12145

[4] MUTATION OF A SITE OF TYROSINE PHOSPHORYLATION IN THE LYMPHOCYTE-SPECIFIC TYROSINE PROTEIN-KINASE, P56LCK, REVEALS ITS ONCOGENIC POTENTIAL IN FIBROBLASTS [J].

AMREIN, KE ;

SEFTON, BM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (12) :4247-4251

[5]

Ausubel FM., 1995, MOL REPROD DEV, V3rd edn, DOI DOI 10.1002/MRD.1080010210

[6] 5'-TERMINAL CAP STRUCTURE IN EUKARYOTIC MESSENGER RIBONUCLEIC-ACIDS [J].

BANERJEE, AK .

MICROBIOLOGICAL REVIEWS, 1980, 44 (02) :175-205

[7]

BONNEAU AM, 1987, J BIOL CHEM, V262, P11134

[8] CDC33 ENCODES MESSENGER-RNA CAP-BINDING PROTEIN EIF-4E OF SACCHAROMYCES-CEREVISIAE [J].

BRENNER, C ;

NAKAYAMA, N ;

GOEBL, M ;

TANAKA, K ;

TOHE, A ;

MATSUMOTO, K .

MOLECULAR AND CELLULAR BIOLOGY, 1988, 8 (08) :3556-3559

[9] DIFFERENTIAL EFFICIENCIES OF INVITRO TRANSLATION OF MOUSE C-MYC TRANSCRIPTS DIFFERING IN THE 5' UNTRANSLATED REGION [J].

DARVEAU, A ;

PELLETIER, J ;

SONENBERG, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (08) :2315-2319

[10]

DUNCAN R, 1987, J BIOL CHEM, V262, P380

← 1 2 3 4 →