MALIGNANT TRANSFORMATION BY A EUKARYOTIC INITIATION-FACTOR SUBUNIT THAT BINDS TO MESSENGER-RNA 5' CAP

被引：849

作者：

LAZARISKARATZAS, A ^{[1
]}

MONTINE, KS ^{[1
]}

SONENBERG, N ^{[1
]}

机构：

[1] MCGILL UNIV,MCGILL CANC CTR,MONTREAL H3G 1Y6,QUEBEC,CANADA

来源：

NATURE | 1990年 / 345卷 / 6275期

关键词：

D O I：

10.1038/345544a0

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

EUKARYOTIC cellular mRNAs have a 5' cap structure (m7 GpppX) that facilitates binding to ribosomes and is required for efficient translation1-3. A specific initiation factor, eIF-4F, mediates the function of the cap4-6 and consists of three subunits7-10, one of which, eIF-4E, binds the cap. This subunit is present in limiting amounts in the cell11-13, and is thought to be regulated by phosphorylation12-16: decreased phosphorylation of eIF-4E following various treatments correlates with a decrease in cellular translation rate. These observations suggest that eIF-4E lies on the mitogenic signal transduction pathway, and we reasoned that overexpression of eIF-4E might profoundly affect cellular growth properties. We report here that overexpression of eIF-4E in NIH 3T3 and Rat 2 fibroblasts causes their tumorigenic transformation as determined by three criteria: formation of transformed foci on a monolayer of cells; anchorage-independent growth; and tumour formation in nude mice. © 1990 Nature Publishing Group.

引用

页码：544 / 547

页数：4