Experimental tests to definitively determine ubiquitylation of a substrate

被引：34

作者：

Bloom, J

Pagano, M

机构：

[1] NYU, Sch Med, Dept Pathol, New York, NY 10012 USA

[2] NYU, Inst Canc, New York, NY USA

来源：

UBIQUITIN AND PROTEIN DEGRADATION, PT B | 2005年 / 399卷

关键词：

D O I：

10.1016/S0076-6879(05)99017-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Ubiquitin-mediated proteolysis is a major pathway of protein degradation that regulates numerous cellular processes. An understanding of the circumstances that contribute to the ubiquitylation of a specific protein can yield vast insight into its regulation. This article examines multiple procedures that explain whether a protein is ubiquitylated and suggests methods to investigate the factors that specifically target the substrate for ubiquitylation, as well as the site of ubiquitin conjugation.

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页码：249 / 266

页数：18

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