A new method for identifying the origins of simple and complex hopanoids in sedimentary materials using stable isotope labelling with 13CH4 and compound specific stable isotope analyses

被引：25

作者：

Crossman, ZM

McNamara, N

Parekh, N

Ineson, P

Evershed, RP

机构：

[1] Univ Bristol, Sch Chem, Organ Geochem Unit, Bristol BS8 1TS, Avon, England

[2] Ctr Ecol & Hydrol, Merlewood Res Stn, Grange Over Sands LA11 6JU, Cumbria, England

[3] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England

来源：

ORGANIC GEOCHEMISTRY | 2001年 / 32卷 / 02期

关键词：

pentacyclic triterpenoid; hopanoid; methanotrophic bacteria; C-13-labelled methane; gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS);

D O I：

10.1016/S0146-6380(00)00178-9

中图分类号：

P3 [地球物理学]; P59 [地球化学];

学科分类号：

0708 ; 070902 ;

摘要：

Forest soils were taken and incubated with C-13-labelled methane. The lipids from these soils were isolated using a Bligh Dyer extraction and fractionated. Simple hopanoids were present in the neutral fraction, while periodic acid/sodium borohydride treatment of the total lipid extract released primary alcohols from polyfunctionalised bacteriohopane derivatives. All hopanoids were analysed by GC, GC/MS and GC/C/IRMS. Specific hopanoids, namely: diploptene, 17 beta (H), 21 beta (H)-homohopanol and 17 beta (H), 21 beta (H)-bishomohopanol produced by methane oxidising bacteria were significantly enriched in C-13 whilst others showed no enrichment. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：359 / 364

页数：6

共 17 条

[1] KINETICS OF CH4 OXIDATION IN OXIC SOILS EXPOSED TO AMBIENT AIR OR HIGH CH4 MIXING RATIOS [J].