A new method for identifying the origins of simple and complex hopanoids in sedimentary materials using stable isotope labelling with 13CH4 and compound specific stable isotope analyses

被引:25
作者
Crossman, ZM
McNamara, N
Parekh, N
Ineson, P
Evershed, RP
机构
[1] Univ Bristol, Sch Chem, Organ Geochem Unit, Bristol BS8 1TS, Avon, England
[2] Ctr Ecol & Hydrol, Merlewood Res Stn, Grange Over Sands LA11 6JU, Cumbria, England
[3] Univ York, Dept Biol, York YO10 5YW, N Yorkshire, England
关键词
pentacyclic triterpenoid; hopanoid; methanotrophic bacteria; C-13-labelled methane; gas chromatography/combustion/isotope ratio mass spectrometry (GC/C/IRMS);
D O I
10.1016/S0146-6380(00)00178-9
中图分类号
P3 [地球物理学]; P59 [地球化学];
学科分类号
0708 ; 070902 ;
摘要
Forest soils were taken and incubated with C-13-labelled methane. The lipids from these soils were isolated using a Bligh Dyer extraction and fractionated. Simple hopanoids were present in the neutral fraction, while periodic acid/sodium borohydride treatment of the total lipid extract released primary alcohols from polyfunctionalised bacteriohopane derivatives. All hopanoids were analysed by GC, GC/MS and GC/C/IRMS. Specific hopanoids, namely: diploptene, 17 beta (H), 21 beta (H)-homohopanol and 17 beta (H), 21 beta (H)-bishomohopanol produced by methane oxidising bacteria were significantly enriched in C-13 whilst others showed no enrichment. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:359 / 364
页数:6
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