Printing protein arrays from DNA arrays

被引：101

作者：

He, Mingyue ^{[2
]}

Stoevesandt, Oda ^{[2
]}

Palmer, Elizabeth A. ^{[2
]}

Khan, Farid ^{[2
]}

Ericsson, Olle ^{[1
]}

Taussig, Michael J. ^{[2
]}

机构：

[1] Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, SE-75185 Uppsala, Sweden

[2] Babraham Inst, Technol Res Grp, Cambridge CB22 3AT, England

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH.1178

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.

引用

页码：175 / 177

页数：3

共 16 条

[1] Generation of high density protein microarrays by cell-free in situ expression of unpurified PCR products [J].

Angenendt, Philipp ;

Kreutzberger, Juergen ;

Gloekler, Joern ;

Hoheisel, Joerg D. .

MOLECULAR & CELLULAR PROTEOMICS, 2006, 5 (09) :1658-1666

[2] Advances in functional protein microarray technology [J].

Bertone, P ;

Snyder, M .

FEBS JOURNAL, 2005, 272 (21) :5400-5411

[3] Glycosylation is essential for translocation of carp retinol-binding protein across the endoplasmic reticulum membrane [J].

Devirgillis, C ;

Gaetani, S ;

Apreda, M ;

Bellovino, D .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2005, 332 (02) :504-511

[4]

HE M, IN PRESS CURR OPIN B

[5] Single step generation of protein arrays from DNA by cell-free expression and in situ immobilisation (PISA method) [J].

He, MY ;

Taussig, MJ .

NUCLEIC ACIDS RESEARCH, 2001, 29 (15)

[6] Profiling humoral autoimmune repertoire of dilated cardiomyopathy (DCM) patients and development of a disease-associated protein chip [J].

Horn, S ;

Lueking, A ;

Murphy, D ;

Staudt, A ;

Gutjahr, C ;

Schulte, K ;

König, A ;

Landsberger, M ;

Lehrach, H ;

Felix, SB ;

Cahill, DJ .

PROTEOMICS, 2006, 6 (02) :605-613

[7] Double-hexahistidine tag with high-affinity binding for protein immobilization, purification, and detection on Ni-nitrilotriacetic acid surfaces [J].

Khan, Farid ;

He, Mingyue ;

Taussig, Michael J. .

ANALYTICAL CHEMISTRY, 2006, 78 (09) :3072-3079

[8] A systematic approach for testing expression of human full-length proteins in cell-free expression systems [J].

Langlais, Claudia ;

Guilleaume, Birgit ;

Wermke, Nadja ;

Scheuermann, Tina ;

Ebert, Lars ;

LaBaer, Joshua ;

Korn, Bernhard .

BMC BIOTECHNOLOGY, 2007, 7 (1)

[9]

MacBeath G, 2000, SCIENCE, V289, P1760

[10] A highly flexible tRNA acylation method for non-natural potypeptide synthesis [J].

Murakami, H ;

Ohta, A ;

Ashigai, H ;

Suga, H .

NATURE METHODS, 2006, 3 (05) :357-359

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