Printing protein arrays from DNA arrays

被引：101

作者：

He, Mingyue ^{[2
]}

Stoevesandt, Oda ^{[2
]}

Palmer, Elizabeth A. ^{[2
]}

Khan, Farid ^{[2
]}

Ericsson, Olle ^{[1
]}

Taussig, Michael J. ^{[2
]}

机构：

[1] Uppsala Univ, Rudbeck Lab, Dept Genet & Pathol, SE-75185 Uppsala, Sweden

[2] Babraham Inst, Technol Res Grp, Cambridge CB22 3AT, England

来源：

NATURE METHODS | 2008年 / 5卷 / 02期

关键词：

D O I：

10.1038/NMETH.1178

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a method, DNA array to protein array (DAPA), which allows the 'printing' of replicate protein arrays directly from a DNA array template using cell-free protein synthesis. At least 20 copies of a protein array can be obtained from a single DNA array. DAPA eliminates the need for separate protein expression, purification and spotting, and also overcomes the problem of long-term functional storage of surface-bound proteins.

引用

页码：175 / 177

页数：3

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