Identification of Arabidopsis rat mutants

被引:115
作者
Zhu, YM
Nam, J
Humara, JM
Mysore, KS
Lee, LY
Cao, HB
Valentine, L
Li, JL
Kaiser, AD
Kopecky, AL
Hwang, HH
Bhattacharjee, S
Rao, PK
Tzfira, T
Rajagopal, J
Yi, HC
Veena
Yadav, BS
Crane, YM
Lin, K
Larcher, Y
Gelvin, MJK
Knue, M
Ramos, C
Zhao, XW
Davis, SJ
Kim, SI
Ranjith-Kumar, CT
Choi, YJ
Hallan, VK
Chattopadhyay, S
Sui, XZ
Ziemienowicz, A
Matthysse, AG
Citovsky, V
Hohn, B
Gelvin, SB [1 ]
机构
[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[2] Dong A Univ, Fac Nat Resources & Life Sci, Pusan 604714, South Korea
[3] Friedrich Miescher Inst, CH-4002 Basel, Switzerland
[4] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
[5] Jagiellonian Univ, Fac Biotechnol, PL-30387 Krakow, Poland
[6] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
关键词
D O I
10.1104/pp.103.020420
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.
引用
收藏
页码:494 / 505
页数:12
相关论文
共 44 条
[1]   Nuclear localization signal binding protein from Arabidopsis mediates nuclear import of Agrobacterium VirD2 protein [J].
Ballas, N ;
Citovsky, V .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (20) :10723-10728
[2]   BOTERO1 is required for normal orientation of cortical microtubules and anisotropic cell expansion in Arabidopsis [J].
Bichet, A ;
Desnos, T ;
Turner, S ;
Grandjean, O ;
Höfte, H .
PLANT JOURNAL, 2001, 25 (02) :137-148
[3]   T-DNA integration into the Arabidopsis genome depends on sequences of pre-insertion sites [J].
Brunaud, V ;
Balzergue, S ;
Dubreucq, B ;
Aubourg, S ;
Samson, F ;
Chauvin, S ;
Bechtold, N ;
Cruaud, C ;
DeRose, R ;
Pelletier, G ;
Lepiniec, L ;
Caboche, M ;
Lecharny, A .
EMBO REPORTS, 2002, 3 (12) :1152-1157
[4]   GENES ENCODING A HISTONE H3.3-LIKE VARIANT IN ARABIDOPSIS CONTAIN INTERVENING SEQUENCES [J].
CHAUBET, N ;
CLEMENT, B ;
GIGOT, C .
JOURNAL OF MOLECULAR BIOLOGY, 1992, 225 (02) :569-574
[5]   Tissue-dependent enhancement of transgene expression by introns of replacement histone H3 genes of Arabidopsis [J].
Chaubet-Gigot, N ;
Kapros, T ;
Flenet, M ;
Kahn, K ;
Gigot, C ;
Waterborg, JH .
PLANT MOLECULAR BIOLOGY, 2001, 45 (01) :17-30
[6]   Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells [J].
Christie, PJ ;
Vogel, JP .
TRENDS IN MICROBIOLOGY, 2000, 8 (08) :354-360
[7]   Floral dip:: a simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana [J].
Clough, SJ ;
Bent, AF .
PLANT JOURNAL, 1998, 16 (06) :735-743
[8]   AGROBACTERIUM-MEDIATED TRANSFORMATION OF GERMINATING-SEEDS OF ARABIDOPSIS-THALIANA - A NON-TISSUE CULTURE APPROACH [J].
FELDMANN, KA ;
MARKS, MD .
MOLECULAR AND GENERAL GENETICS, 1987, 208 (1-2) :1-9
[9]   T-DNA INSERTION MUTAGENESIS IN ARABIDOPSIS - PROSPECTS AND PERSPECTIVES [J].
FORSTHOEFEL, NR ;
WU, YW ;
SCHULZ, B ;
BENNETT, MJ ;
FELDMANN, KA .
AUSTRALIAN JOURNAL OF PLANT PHYSIOLOGY, 1992, 19 (04) :353-366
[10]   Agrobacterium and plant genes involved in T-DNA transfer and integration [J].
Gelvin, SB .
ANNUAL REVIEW OF PLANT PHYSIOLOGY AND PLANT MOLECULAR BIOLOGY, 2000, 51 :223-256