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Identification of Arabidopsis rat mutants

被引：115
作者：
Zhu, YM
Nam, J
Humara, JM
Mysore, KS
Lee, LY
Cao, HB
Valentine, L
Li, JL
Kaiser, AD
Kopecky, AL
Hwang, HH
Bhattacharjee, S
Rao, PK
Tzfira, T
Rajagopal, J
Yi, HC
Veena
Yadav, BS
Crane, YM
Lin, K
Larcher, Y
Gelvin, MJK
Knue, M
Ramos, C
Zhao, XW
Davis, SJ
Kim, SI
Ranjith-Kumar, CT
Choi, YJ
Hallan, VK
Chattopadhyay, S
Sui, XZ
Ziemienowicz, A
Matthysse, AG
Citovsky, V
Hohn, B
Gelvin, SB [1 ]
机构：
[1] Purdue Univ, Dept Biol Sci, W Lafayette, IN 47907 USA
[2] Dong A Univ, Fac Nat Resources & Life Sci, Pusan 604714, South Korea
[3] Friedrich Miescher Inst, CH-4002 Basel, Switzerland
[4] SUNY Stony Brook, Dept Biochem & Cell Biol, Stony Brook, NY 11794 USA
[5] Jagiellonian Univ, Fac Biotechnol, PL-30387 Krakow, Poland
[6] Univ N Carolina, Dept Biol, Chapel Hill, NC 27599 USA
来源：
PLANT PHYSIOLOGY | 2003年 / 132卷 / 02期
关键词：
D O I：
10.1104/pp.103.020420
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
Limited knowledge currently exists regarding the roles of plant genes and proteins in the Agrobacterium tumefaciens-mediated transformation process. To understand the host contribution to transformation, we carried out root-based transformation assays to identify Arabidopsis mutants that are resistant to Agrobacterium transformation (rat mutants). To date, we have identified 126 rat mutants by screening libraries of T-DNA insertion mutants and by using various "reverse genetic" approaches. These mutants disrupt expression of genes of numerous categories, including chromatin structural and remodeling genes, and genes encoding proteins implicated in nuclear targeting, cell wall structure and metabolism, cytoskeleton structure and function, and signal transduction. Here, we present an update on the identification and characterization of these rat mutants.
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收藏
页码：494 / 505
页数：12
相关论文
共 44 条
[21]   EFFICIENT ISOLATION AND MAPPING OF ARABIDOPSIS-THALIANA T-DNA INSERT JUNCTIONS BY THERMAL ASYMMETRIC INTERLACED PCR [J].
LIU, YG ;
MITSUKAWA, N ;
OOSUMI, T ;
WHITTIER, RF .
PLANT JOURNAL, 1995, 8 (03) :457-463
[22]   CHARACTERIZATION OF NONATTACHING MUTANTS OF AGROBACTERIUM-TUMEFACIENS [J].
MATTHYSSE, AG .
JOURNAL OF BACTERIOLOGY, 1987, 169 (01) :313-323
[23]   SEQUENCE-BASED IDENTIFICATION OF T-DNA INSERTION MUTATIONS IN ARABIDOPSIS - ACTIN MUTANTS ACT2-1 AND ACT4-1 [J].
MCKINNEY, EC ;
ALI, N ;
TRAUT, A ;
FELDMANN, KA ;
BELOSTOTSKY, DA ;
MCDOWELL, JM ;
MEAGHER, RB .
PLANT JOURNAL, 1995, 8 (04) :613-622
[24]   Role of the Agrobacterium tumefaciens VirD2 protein in T-DNA transfer and integration [J].
Mysore, KS ;
Bassuner, B ;
Deng, XB ;
Darbinian, NS ;
Motchoulski, A ;
Ream, W ;
Gelvin, SB .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (07) :668-683
[25]   An Arabidopsis histone H2A mutant is deficient in Agrobacterium T-DNA integration [J].
Mysore, KS ;
Nam, J ;
Gelvin, SB .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (02) :948-953
[26]   Arabidopsis ecotypes and mutants that are recalcitrant to Agrobacterium root transformation are susceptible to germ-line transformation [J].
Mysore, KS ;
Kumar, CTR ;
Gelvin, SB .
PLANT JOURNAL, 2000, 21 (01) :9-16
[27]   Identification of T-DNA tagged Arabidopsis mutants that are resistant to transformation by Agrobacterium [J].
Nam, J ;
Mysore, KS ;
Zheng, C ;
Knue, MK ;
Matthysse, AG ;
Gelvin, SB .
MOLECULAR AND GENERAL GENETICS, 1999, 261 (03) :429-438
[28]   Differences in susceptibility of Arabidopsis ecotypes to crown gall disease may result from a deficiency in T-DNA integration [J].
Nam, J ;
Matthysse, AG ;
Gelvin, SB .
PLANT CELL, 1997, 9 (03) :317-333
[29]   Agrobacterium tumefaciens transformation of the radiation hypersensitive Arabidopsis thaliana mutants uvh1 and rad5 [J].
Nam, J ;
Mysore, KS ;
Gelvin, SB .
MOLECULAR PLANT-MICROBE INTERACTIONS, 1998, 11 (11) :1136-1141
[30]   Early transcription of Agrobacterium T-DNA genes in tobacco and maize [J].
Narasimhulu, SB ;
Deng, X ;
Sarria, R ;
Gelvin, SB .
PLANT CELL, 1996, 8 (05) :873-886
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